Tris(hydroxymethyl)aminomethane-functionalized agarose particles: Parameters affecting the binding of bovine serum albumin

被引：8

作者：

Zhang, Bin ^{[1
,2
,3
]}

Wang, Ye ^{[4
]}

Gao, Min ^{[1
]}

Gu, Ming ^{[1
]}

Wang, Changhai ^{[2
,4
]}

机构：

[1] Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China

[3] Chinese Acad Sci, Grad Univ, Beijing 100190, Peoples R China

[4] Nanjing Agr Univ, Nanjing, Jiangsu, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2012年 / 35卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Adsorption equilibrium; Bovine serum albumin; Protein adsorption; Sepharose HP; Tris(hydroxymethyl)aminomethane; CHARGE INDUCTION CHROMATOGRAPHY; CHELATING RESINS; LIGAND;

D O I：

10.1002/jssc.201200118

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new protein adsorbent is introduced based on the coupling of the common buffer ion, tris(hydroxymethyl)aminomethane, to the agarose gel Sepharose HP from GE Healthcare Bio-Sciences AB, Uppsala, Sweden. The article describes the synthesis of the new adsorbent and the use of BSA as a model in a binding study. By optimization of the coupling procedure, a maximum ligand density of 63.5 mu mol/mL gel could be obtained. Adsorption equilibria were investigated in the pH range 5.0-8.0 and at salt concentrations of 0-0.4 mol/L. Binding of BSA under different conditions indicated that both electrostatic interaction and hydrogen bonding were involved in the adsorption process where the former played a major role.

引用

页码：1406 / 1410

页数：5

共 16 条

[1] Chemically modified activated carbon with tris(hydroxymethyl) aminomethane for selective adsorption and determination of gold in water samples [J].

Albishri, Hassan M. ;

Marwani, Hadi M. .

ARABIAN JOURNAL OF CHEMISTRY, 2016, 9 :S252-S258

[2] Tris(hydroxymethyl)aminomethane-functionalized silica particles and their application for hydrophilic interaction chromatography [J].

Bui, Nhat Thi Hong ;

Verhage, Jeroen J. ;

Irgum, Knut .

JOURNAL OF SEPARATION SCIENCE, 2010, 33 (19) :2965-2976

[3] H++:: a server for estimating pKas and adding missing hydrogens to macromolecules [J].

Gordon, JC ;

Myers, JB ;

Folta, T ;

Shoja, V ;

Heath, LS ;

Onufriev, A .

NUCLEIC ACIDS RESEARCH, 2005, 33 :W368-W371

[4]

Jyo A, 2001, ANAL SCI, V17, P1211

[5] A mild hydrophobic interaction chromatography involving polyethylene glycol immobilized to agarose media refolding recombinant Staphylococcus aureus elongation factor G [J].

Li, JJ ;

Venkataramana, M ;

Wang, AQ ;

Sanyal, S ;

Janson, JC ;

Su, ZG .

PROTEIN EXPRESSION AND PURIFICATION, 2005, 40 (02) :327-335

[6] PREPARATION OF CHELATING RESINS SELECTIVE TO BORIC-ACID BY FUNCTIONALIZATION OF MACROPOROUS POLY(GLYCIDYL METHACRYLATE) WITH 2-AMINO-2-HYDROXYMETHYL-1,3-PROPANEDIOL [J].

MAEDA, H ;

EGAWA, H ;

JYO, A .

SEPARATION SCIENCE AND TECHNOLOGY, 1995, 30 (18) :3545-3554

[7] CHROMATOGRAPHIC ISOLATION OF 80S RIBOSOMES FROM RAT LIVER AND MOUSE PLASMA CELL TUMOR [J].

PETERSON, EA ;

KUFF, EL .

BIOCHEMISTRY, 1969, 8 (07) :2916-&

[8] AGAR DERIVATIVES FOR CHROMATOGRAPHY, ELECTROPHORESIS AND GEL-BOUND ENZYMES .1. DESULPHATED AND REDUCED CROSS-LINKED AGAR AND AGAROSE IN SPHERICAL BEAD FORM [J].

PORATH, J ;

JANSON, JC ;

LAAS, T .

JOURNAL OF CHROMATOGRAPHY, 1971, 60 (02) :167-&

[9] Synthesis and borate uptake of two novel chelating resins [J].

Qi, T ;

Sonoda, A ;

Makita, Y ;

Kanoh, H ;

Ooi, K ;

Hirotsu, T .

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 2002, 41 (02) :133-138

[10] Highly efficient and low-cost purification of lysozyme: A novel tris(hydroxymethyl)aminomethane immobilized affinity column [J].

Quan, Li ;

Cao, Qing ;

Li, Zhiyu ;

Li, Na ;

Li, Kean ;

Liu, Feng .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2009, 877 (07) :594-598

← 1 2 →