The Non-homologous End-Joining (NHEJ) Mathematical Model for the Repair of Double-Strand Breaks: II. Application to Damage Induced by Ultrasoft X Rays and Low-Energy Electrons

被引：46

作者：

Taleei, Reza ^{[1
]}

Girard, Peter M. ^{[1
]}

Sankaranarayanan, Krishnaswami ^{[1
]}

Nikjoo, Hooshang ^{[1
]}

机构：

[1] Karolinska Inst, Dept Oncol Pathol, Radiat Biophys Grp, SE-17176 Stockholm, Sweden

来源：

RADIATION RESEARCH | 2013年 / 179卷 / 05期

关键词：

HAMSTER V79-4 CELLS; DNA-DAMAGE; DIELECTRIC RESPONSE; LIQUID WATER; HETEROCHROMATIN; RADIATION; PHOSPHORYLATION; INDUCTION; DYNAMICS; TARGETS;

D O I：

10.1667/RR3124.1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We investigated the kinetics of simple and complex types of double-strand breaks (DSB) using our newly proposed mechanistic mathematical model for NHEJ DSB repair. For this purpose the simulated initial spectrum of DNA DSB, induced in an atomistic canonical model of B-DNA by low-energy single electron tracks, 100 eV to 4.55 keV, and the electrons generated by ultrasoft X rays (C-K, Al-K and Ti-K), were subjected to NHEJ repair processes. The activity elapsed time of sequentially independent steps of repair performed by proteins involved in NHEJ repair process were calculated for separate DSB. The repair kinetics of DSBs were computed and compared with published data on repair kinetics obtained by pulsed-field gel electrophoresis method. The comparison shows good agreement for V79-4 cells irradiated with ultrasoft X rays. The average times for the repair of simple and complex DSB confirm that double-strand break complexity is a potential explanation for the slow component of DSB repair observed in V79-4 cells irradiated by ultrasoft X rays. (C) 2013 by Radiation Research Society

引用

页码：540 / 548

页数：9

共 41 条

[1] IN CHEF ELECTROPHORESIS A LINEAR INDUCTION OF DSB CORRESPONDS TO A NONLINEAR FRACTION OF EXTRACTED DNA WITH DOSE [J].

BLOCHER, D .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1990, 57 (01) :7-12

[2] Induction and rejoining of DNA double-strand breaks in Chinese hamster V79-4 cells irradiated with characteristic aluminum K and copper L ultrasoft X rays [J].

Botchway, SW ;

Stevens, DL ;

Hill, MA ;

Jenner, TJ ;

ONeill, P .

RADIATION RESEARCH, 1997, 148 (04) :317-324

[3] CALCULATION OF INITIAL YIELDS OF SINGLE-STRAND AND DOUBLE-STRAND BREAKS IN CELL-NUCLEI FROM ELECTRONS, PROTONS AND ALPHA-PARTICLES [J].

CHARLTON, DE ;

NIKJOO, H ;

HUMM, JL .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1989, 56 (01) :1-19

[4] Double-Strand Breaks in Heterochromatin Move Outside of a Dynamic HP1a Domain to Complete Recombinational Repair [J].

Chiolo, Irene ;

Minoda, Aki ;

Colmenares, Serafin U. ;

Polyzos, Aris ;

Costes, Sylvain V. ;

Karpen, Gary H. .

CELL, 2011, 144 (05) :732-744

[5] Spatiotemporal characterization of ionizing radiation induced DNA damage foci and their relation to chromatin organization [J].

Costes, S. V. ;

Chiolo, I. ;

Pluth, J. M. ;

Barcellos-Hoff, M. H. ;

Jakob, B. .

MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2010, 704 (1-3) :78-87

[6]

de Lara CM, 2001, RADIAT RES, V155, P440, DOI 10.1667/0033-7587(2001)155[0440:DOTYOD]2.0.CO

[7]

[8] A complete dielectric response model for liquid water: A solution of the Bethe Ridge problem [J].

Emfietzoglou, D ;

Cucinotta, FA ;

Nikjoo, H .

RADIATION RESEARCH, 2005, 164 (02) :202-211

[9] Electron inelastic mean free paths in biological matter based on dielectric theory and local-field corrections [J].

Emfietzoglou, D. ;

Kyriakou, I. ;

Abril, I. ;

Garcia-Molina, R. ;

Petsalakis, I. D. ;

Nikjoo, H. ;

Pathak, A. .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 2009, 267 (01) :45-52

[10] A dielectric response study of the electronic stopping power of liquid water for energetic protons and a new I-value for water [J].

Emfietzoglou, D. ;

Garcia-Molina, R. ;

Kyriakou, I. ;

Abril, I. ;

Nikjoo, H. .

PHYSICS IN MEDICINE AND BIOLOGY, 2009, 54 (11) :3451-3472

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