Enzymatic transformation of ginsenosides Re, Rg1, and Rf to ginsenosides Rg2 and aglycon PPT by using β-glucosidase from Thermotoga neapolitana

被引：13

作者：

Bi, Yun-Feng ^{[1
]}

Wang, Xi-Zhu ^{[1
]}

Jiang, Shan ^{[1
]}

Liu, Jing-Sheng ^{[1
]}

Zheng, Ming-Zhu ^{[1
]}

Chen, Ping ^{[1
]}

机构：

[1] Jilin Agr Univ, Coll Food Sci & Engn, Changchun 130118, Jilin, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2019年 / 41卷 / 4-5期

关键词：

Biotransformation; Escherichia coli; Enzymatic; Genes; Transformation; PANAX-GINSENG; GLYCOSIDE; RH1; RC; BIOTRANSFORMATION; PROTOPANAXATRIOL; CLASSIFICATION; BIOCONVERSION; MICE; RH-1;

D O I：

10.1007/s10529-019-02665-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

ObjectivesTo enzymatically transform protopanaxatriol by using -glucosidase from Thermotoga neapolitana (T. neapolitana) DSM 4359.ResultsRecombinant -glucosidase was purified, which molecular weight was about 79.5kDa. High levels of ginsenoside were obtained using the follow reaction conditions: 2mgml(-1) ginsenoside, 25 U ml(-1) enzyme, 85 degrees C, and pH 5.0. -glucosidase converted ginsenoside Re to Rg2, Rf and Rg1 to APPT completely after 3h under the given conditions, respectively. The enzyme created 1.66mgml(-1) Rg2 from Re with 553mgl(-1) h(-1), 0.85mgml(-1), and 1.01mgml(-1) APPT from Rg1 and Rf with 283 and 316mgl(-1) h(-1) APPT.Conclusions-glucosidase could be useful for the high-yield, rapid, and low-cost preparation of ginsenoside Rg2 from Re, and APPT from the ginsenosides Rg1 and Rf.

引用

页码：613 / 623

页数：11

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