Neutron and X-ray analysis of the Fenna-Matthews-Olson photosynthetic antenna complex from Prosthecochloris aestuarii

被引：4

作者：

Lu, Xun ^{[1
]}

Selvaraj, Brinda ^{[1
]}

Ghimire-Rijal, Sudipa ^{[1
]}

Orf, Gregory S. ^{[2
,3
]}

Meilleur, Flora ^{[1
,4
]}

Blankenship, Robert E. ^{[2
,3
]}

Cuneo, Matthew J. ^{[1
,5
]}

Myles, Dean A. A. ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Neutron Sci Directorate, Oak Ridge, TN 37831 USA

[2] Washington Univ St Louis, Dept Biol, St Louis, MO 63130 USA

[3] Washington Univ St Louis, Dept Chem, St Louis, MO 63130 USA

[4] North Carolina State Univ, Dept Mol & Struct Biochem, Campus Box 7622, Raleigh, NC 27695 USA

[5] St Jude Childrens Res Hosp, Dept Struct Biol, Memphis, TN 38105 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2019年 / 75卷

关键词：

neutron crystallography; photosynthesis; hydrogen; site energy; neutron diffraction; Fenna-Matthews-Olson protein; Prosthecochloris aestuarii; QUANTUM COHERENCE; PROTEIN; BACTERIOCHLOROPHYLL;

D O I：

10.1107/S2053230X19000724

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The Fenna-Matthews-Olson protein from Prosthecochloris aestuarii (PaFMO) has been crystallized in a new form that is amenable to high-resolution X-ray and neutron analysis. The crystals belonged to space group H3, with unit-cell parameters a = b = 83.64, c = 294.78 angstrom, and diffracted X-rays to similar to 1.7 angstrom resolution at room temperature. Large PaFMO crystals grown to volumes of 0.3-0.5 mm(3) diffracted neutrons to 2.2 angstrom resolution on the MaNDi neutron diffractometer at the Spallation Neutron Source. The resolution of the neutron data will allow direct determination of the positions of H atoms in the structure, which are believed to be fundamentally important in tuning the individual excitation energies of bacteriochlorophylls in this archetypal photosynthetic antenna complex. This is one of the largest unit-cell systems yet studied using neutron diffraction, and will allow the first high-resolution neutron analysis of a photosynthetic antenna complex.

引用

页码：171 / 175

页数：5

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