Occurrence of Carbapenemase-Producing Klebsiella pneumoniae in Bat Guano

被引：0
作者：
Gharout-Sait, Alima [1 ]
Touati, Abdelaziz [1 ]
Ahmim, Mourad [2 ]
Brasme, Lucien [3 ,4 ]
Guillard, Thomas [3 ,4 ]
Agsous, Amir [1 ]
de Champs, Christophe [3 ,4 ]
机构：
[1] Univ Bejaia, FSNV, Lab Ecol Microbienne, Bejaia 06000, Algeria
[2] Univ Bejaia, FSNV, Lab Ecol & Environm, Bejaia 06000, Algeria
[3] CHU Reims, Hop Robert Debre, Lab Bacteriol, Virol Hyg Hosp, Ave Gen Koenig, Reims, France
[4] Univ Reims, Inserm UMR S 1250 P3Cell, SFR CAP Sante, Reims, France
来源：
MICROBIAL DRUG RESISTANCE | 2019年 / 25卷 / 07期
关键词：
OXA-48; KPC-3; Klebsiella pneumoniae; bat guano; Algeria; SPECTRUM-BETA-LACTAMASE; ANTIBIOTIC-RESISTANCE; RAPID DETECTION; ENTEROBACTERIACEAE; PLASMID; KPC; IDENTIFICATION; SALMONELLA; BACTERIA; GENE;
D O I：
10.1089/mdr.2018.0471
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The aim of this study was to screen for the presence of carbapenemase-producing Enterobacteriaceae (CPE) isolates from bat guano in Bejaia, Algeria. Guano samples (n = 110) were collected in Aokas's cave, Bejaia, Algeria, between March and May 2016. Samples were plated on MacConkey agar supplemented with ertapenem (0.5 mg/L) and vancomycin (32 mg/L). The isolates were identified and antimicrobial susceptibility was determined using disk diffusion method. Carbapenemase, extended spectrum beta-lactamases, plasmid-mediated AmpC, and plasmid-mediated quinolone resistance genes were studied using PCR and sequencing. Clonal relatedness was studied using multilocus sequence typing (MLST). Two CPE isolates were identified as Klebsiella pneumoniae. PCR and sequencing identified the blaOXA-48 in one K. pneumoniae strain (CS34) and blaKPC-3 in the other strain (CS63). K. pneumoniae CS63 was found to carry bla(TEM-1) and aac(6 ')-Ib genes. The MLST showed that K. pneumoniae CS63 was assigned to ST512, whereas K. pneumoniae CS34 belonged to ST1878. This is the first description of CPE from bats' guano.
引用
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页码：1057 / 1062
页数：6
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