Wheat alkylresorcinols protect human retinal pigment epithelial cells against H2O2-induced oxidative damage through Akt-dependent Nrf2/HO-1 signaling

被引：4

作者：

Wang, Ziyuan ^{[1
]}

Hao, Yiming ^{[1
]}

Wang, Yu ^{[1
]}

Liu, Jie ^{[1
]}

Yuan, Xiaoping ^{[2
]}

Sun, Baoguo ^{[1
]}

Wang, Jing ^{[1
]}

机构：

[1] BTBU, China Canada Joint Lab Food Nutr & Hlth Beijing, Beijing 100048, Peoples R China

[2] China Grain Reserves Corp, Dept Sci & Technol, Beijing 100039, Peoples R China

来源：

FOOD & FUNCTION | 2019年 / 10卷 / 05期

关键词：

CHOLESTEROL EXCRETION; VITAMIN-A; ACTIVATION; STRESS; NRF2;

D O I：

10.1039/c8fo02564a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The protective effect of wheat alkylresorcinols (ARs) on human retinal pigment epithelium cells (ARPE-19) against oxidative stress and the possible underlying mechanism were investigated in this study. The results showed that ARs significantly inhibited 300 M H2O2-induced ARPE-19 cell damage and reactive oxygen species (ROS) generation by 19% and 32%, respectively. Moreover, ARs treatment increased NF-E2-related factor 2 (Nrf2) signaling activation, which was evidenced by increased transcription of anti-oxidant responsive genes GCL, NQO1 and HO-1. Knockdown of Nrf2 through targeted siRNA alleviated ARs-mediated HO-1 transcription, and almost abolished ARs-mediated cytoprotection against H2O2 induced cell damage. Further studies showed that the protective effect of ARs was dependent on Akt activation. Taken together, these results demonstrated that ARs could protect ARPE-19 cells from oxidative stress induced cell damage possibly through Akt dependent Nrf2/HO-1 signaling.

引用

页码：2797 / 2804

页数：8

共 18 条

[1] Effects of increased wholegrain consumption on immune and inflammatory markers in healthy low habitual wholegrain consumers [J].

Ampatzoglou, Antonios ;

Williams, Charlotte L. ;

Atwal, Kiranjit K. ;

Maidens, Catherine M. ;

Ross, Alastair B. ;

Thielecke, Frank ;

Jonnalagadda, Satya S. ;

Kennedy, Orla B. ;

Yaqoob, Parveen .

EUROPEAN JOURNAL OF NUTRITION, 2016, 55 (01) :183-195

[2] Adverse events and complications associated with intravitreal injection of anti-VEGF agents: a review of literature [J].

Falavarjani, K. Ghasemi ;

Nguyen, Q. D. .

EYE, 2013, 27 (07) :787-794

[3] Lutein Activates the Transcription Factor Nrf2 in Human Retinal Pigment Epithelial Cells [J].

Frede, Katja ;

Ebert, Franziska ;

Kipp, Anna P. ;

Schwerdtle, Tanja ;

Baldermann, Susanne .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 65 (29) :5944-5952

[4] Effects of region and cultivar on alkylresorcinols content and composition in wheat bran and their antioxidant activity [J].

Gunenc, Aynur ;

HadiNezhad, Mehri ;

Tamburic-Ilincic, Lily ;

Mayer, Paul M. ;

Hosseinian, Farah .

JOURNAL OF CEREAL SCIENCE, 2013, 57 (03) :405-410

[5] Sodium tanshinone IIA sulfonate protects ARPE-19 cells against oxidative stress by inhibiting autophagy and apoptosis [J].

Han, Dongmei ;

Wu, Xingwei ;

Liu, Libin ;

Shu, Wanting ;

Huang, Zhenping .

SCIENTIFIC REPORTS, 2018, 8

[6] Wheat alkylresorcinols reduce micellar solubility of cholesterol in vitro and increase cholesterol excretion in mice [J].

Horikawa, Kazumasa ;

Hashimoto, Chiaki ;

Kikuchi, Yosuke ;

Makita, Miki ;

Fukudome, Shin-ichi ;

Okita, Kimiko ;

Wada, Naoyuki ;

Oishi, Katsutaka .

NATURAL PRODUCT RESEARCH, 2017, 31 (05) :578-582

[7] Berberine activates Nrf2 nuclear translocation and protects against oxidative damage via a phosphatidylinositol 3-kinase/Akt-dependent mechanism in NSC34 motor neuron-like cells [J].

Hsu, Ya-Yun ;

Chen, Cheng-Sheng ;

Wu, Sheng-Nan ;

Jong, Yuh-Jyh ;

Lo, Yi-Ching .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2012, 46 (05) :415-425

[8] Carotenoids, inflammation, and oxidative stress-implications of cellular signaling pathways and relation to chronic disease prevention [J].

Kaulmann, Anouk ;

Bohn, Torsten .

NUTRITION RESEARCH, 2014, 34 (11) :907-929

[9] Associations between fruits, vegetables, vitamin A, β-carotene and flavonol dietary intake, and age-related macular degeneration in elderly women in Korea: the Fifth Korea National Health and Nutrition Examination Survey [J].

Kim, E-k ;

Kim, H. ;

Kwon, O. ;

Chang, N. .

EUROPEAN JOURNAL OF CLINICAL NUTRITION, 2018, 72 (01) :161-167

[10] Identification, Quantification, and Anti-inflammatory Activity of 5-n-Alkylresorcinols from 21 Different Wheat Varieties [J].

Liu, Jie ;

Hao, Yiming ;

Wang, Ziyuan ;

Ni, Fang ;

Wang, Yu ;

Gong, Lingxiao ;

Sun, Baoguo ;

Wang, Jing .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2018, 66 (35) :9241-9247

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