Production and Partial Characterization of α-Amylase Enzyme from Bacillus sp BCC 01-50 and Potential Applications

被引：67

作者：

Simair, Altaf Ahmed ^{[1
]}

Qureshi, Abdul Sattar ^{[2
]}

Khushk, Imrana ^{[2
]}

Ali, Chaudhry Haider ^{[3
,4
,5
]}

Lashari, Safia ^{[2
]}

Bhutto, Muhammad Aqeel ^{[1
,2
]}

Mangrio, Ghulam Sughra ^{[2
]}

Lu, Changrui ^{[1
]}

机构：

[1] Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China

[2] Univ Sindh, Inst Biotechnol & Genet Engn, Jamshoro 76080, Pakistan

[3] Univ Engn & Technol, Dept Chem Engn, KSK Campus, Lahore 54890, Pakistan

[4] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[5] East China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2017年 / 2017卷

基金：

中国博士后科学基金;

关键词：

SOLID-STATE FERMENTATION; RAW STARCH; PURIFICATION; OPTIMIZATION; EXPRESSION;

D O I：

10.1155/2017/9173040

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Amylase is an industrially important enzyme and applied in many industrial processes such as saccharification of starchy materials, food, pharmaceutical, detergent, and textile industries. This research work deals with the optimization of fermentation conditions for alpha-amylase production from thermophilic bacterial strain Bacillus sp. BCC 01-50 and characterization of crude amylase. The time profile of bacterial growth and amylase production was investigated in synthetic medium and maximum enzyme titer was observed after 60 h. In addition, effects of different carbon sources were tested as a substrate for amylase production and molasses was found to be the best. Various organic and inorganic compounds, potassium nitrate, ammonium chloride, sodium nitrate, urea, yeast extract, tryptone, beef extract, and peptone, were used and beef extract was found to be the best among the nitrogen sources used. Temperature, pH, agitation speed, and size of inoculum were also optimized. Highest enzyme activity was obtained when the strain was cultured in molasses medium for 60 h in shaking incubator (150 rpm) at 50 degrees C and pH 8. Crude amylase showed maximal activity at pH 9 and 65 degrees C. Enzyme remained stable in alkaline pH range 9-10 and 60-70 degrees C. Crude amylase showed great potential for its application in detergent industry and saccharification of starchy materials.

引用

页数：9

共 28 条

[1]

Abd-Elhalem B. T., 2015, Annals of Agricultural Science (Cairo), V60, P193, DOI 10.1016/j.aoas.2015.06.001

[2]

Abdullah R, 2014, PAK J BOT, V46, P1071

[3]

Asad W, 2011, PAK J BOT, V43, P1045

[4] Studies on α-amylase production by Bacillus licheniformis MIR-61 [J].