Development of a New Amycolatopsis orientalis Strain for High-Yield Eremomycin Production

被引：0

作者：

Dzhavakhiya, V. V. ^{[1
]}

Glagoleva, E. V. ^{[1
]}

Popova, E. D. ^{[1
]}

Ovchinnikov, A. I. ^{[1
]}

Skryabin, K. G. ^{[1
]}

Shobolov, D. L. ^{[2
]}

Balabanyan, V. Yu. ^{[2
]}

Chernobrovkin, M. G. ^{[2
]}

机构：

[1] Russian Acad Sci, Fundamentals Biotechnol Fed Res Ctr, Moscow 117312, Russia

[2] Drugs Technol LLC, Moscow 141400, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2017年 / 53卷 / 08期

关键词：

Amycolatopsis orientalis; eremomycin; glycopeptide antibiotics; secondary metabolites; UV mutagenesis; RED MAN SYNDROME;

D O I：

10.1134/S0003683817080026

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multistep random UV mutagenesis of an eremomycin-producing strain Amycolatopsis orientalis VKPM Ac-1125 with rather low productivity (0.5 g/L) and the further selection of the most high-yield mutant strains have resulted in a highly productive E 13-26 strain. After optimization of the fermentation medium by multifactor analysis, the most efficient composition for the fermentation medium was established, %: glycerol, 8; soybean meals, 2.0; MgCl2, 0.02; KH2PO4, 0.02; KNO3, 0.6; CaCl2, 0.3; skim milk powder, 0.46; and glucose, 0.8. The maximum productivity of this strain on the optimized medium reached 3.42 +/- 0.17 g/L. Therefore, the obtained strain is considered to be very promising for further use in the selection and development of technology for the large-scale eremomycin production.

引用

页码：792 / 799

页数：8

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