Developments in cryo-electron tomography for in situ structural analysis

被引：20

作者：

Dubrovsky, Anna ^{[1
]}

Sorrentino, Simona ^{[1
]}

Harapin, Jan ^{[1
]}

Sapra, K. Tanuj ^{[1
]}

Medalia, Ohad ^{[1
,2
,3
]}

机构：

[1] Univ Zurich, Dept Biochem, CH-8057 Zurich, Switzerland

[2] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel

[3] Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, IL-84105 Beer Sheva, Israel

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2015年 / 581卷

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

Cryo-electron tomography; Cryo-focused-ion beam; Direct electron detector; Phase plate; NUCLEAR-PORE COMPLEX; BEAM-INDUCED MOTION; PHASE-CONTRAST; ELECTRON TOMOGRAPHY; MOLECULAR ARCHITECTURE; 3-DIMENSIONAL STRUCTURE; BIOLOGICAL SPECIMENS; CELLULAR PROCESSES; PROTEIN COMPLEXES; EUKARYOTIC CELLS;

D O I：

10.1016/j.abb.2015.04.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology. Recent advances in computational and analytical tools for cryo-electron tomography have enabled the study of macromolecular structures in their native environment, providing unprecedented insights into cell function. Moreover, the recent implementation of direct electron detectors has progressed cryo-electron tomography to a stage where it can now be applied to the reconstruction of macromolecular structures at high resolutions. Here, we discuss some of the recent technical developments in cryo-electron tomography to reveal structures of macromolecular complexes in their physiological medium, focusing mainly on eukaryotic cells. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：78 / 85

页数：8

共 126 条

[1] THE NUCLEAR LAMINA IS A MESHWORK OF INTERMEDIATE-TYPE FILAMENTS [J].

AEBI, U ;

COHN, J ;

BUHLE, L ;

GERACE, L .

NATURE, 1986, 323 (6088) :560-564

[2] A molecular census of 26S proteasomes in intact neurons [J].

Asano, Shoh ;

Fukuda, Yoshiyuki ;

Beck, Florian ;

Aufderheide, Antje ;

Foerster, Friedrich ;

Danev, Radostin ;

Baumeister, Wolfgang .

SCIENCE, 2015, 347 (6220) :439-442

[3] Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles [J].

Bai, Xiao-chen ;

Fernandez, Israel S. ;

McMullan, Greg ;

Scheres, Sjors H. W. .

ELIFE, 2013, 2

[4] Direct electron detection yields cryo-EM reconstructions at resolutions beyond 3/4 Nyquist frequency [J].

Bammes, Benjamin E. ;

Rochat, Ryan H. ;

Jakana, Joanita ;

Chen, Dong-Hua ;

Chiu, Wah .

JOURNAL OF STRUCTURAL BIOLOGY, 2012, 177 (03) :589-601

[5] Membrane protein structure determination using cryo-electron tomography and 3D image averaging [J].

Bartesaghi, Alberto ;

Subramaniam, Sriram .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2009, 19 (04) :402-407

[6] Nuclear pore complex structure and dynamics revealed by cryoelectron tomography [J].

Beck, M ;

Förster, F ;

Ecke, M ;

Plitzko, JM ;

Melchior, F ;

Gerisch, G ;

Baumeister, W ;

Medalia, O .

SCIENCE, 2004, 306 (5700) :1387-1390

[7] Snapshots of nuclear pore complexes in action captured by cryo-electron tomography [J].

Beck, Martin ;

Lucic, Vladan ;

Foerster, Friedrich ;

Baumeister, Wolfgang ;

Medalia, Ohad .

NATURE, 2007, 449 (7162) :611-615

[8] Visualizing cellular processes at the molecular level by cryo-electron tomography [J].

Ben-Harush, Kfir ;

Maimon, Tal ;

Patla, Israel ;

Villa, Elizabeth ;

Medalia, Ohad .

JOURNAL OF CELL SCIENCE, 2010, 123 (01) :7-12

[9] The Supramolecular Organization of the C. elegans Nuclear Lamin Filament [J].

Ben-Harush, Kfir ;

Wiesel, Naama ;

Frenkiel-Krispin, Daphna ;

Moeller, Dorothee ;

Soreq, Eyal ;

Aebi, Ueli ;

Herrmann, Harald ;

Gruenbaum, Yosef ;

Medalia, Ohad .

JOURNAL OF MOLECULAR BIOLOGY, 2009, 386 (05) :1392-1402

[10] The structure and function of platelet integrins [J].

Bennett, J. S. ;

Berger, B. W. ;

Billings, P. C. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2009, 7 :200-205

← 1 2 3 4 5 6 7 8 9 10 →