[18F]desmethoxyfallypride as a novel PET radiotracer for quantitative in vivo dopamine D2/D3 receptor imaging in rat models of neurodegenerative diseases

被引：12

作者：

Doebroessy, Mate D. ^{[1
]}

Braun, Friederike ^{[3
]}

Klein, Stefanie ^{[4
]}

Garcia, Joanna ^{[2
]}

Langen, Karl-Josef ^{[4
]}

Weber, Wolfgang A. ^{[3
]}

Nikkhah, Guido ^{[1
]}

Meyer, Philipp T. ^{[3
]}

机构：

[1] Univ Freiburg, Med Ctr, Dept Gen Neurosurg, D-79106 Freiburg, Germany

[2] Univ Freiburg, Med Ctr, Dept Neurol, D-79106 Freiburg, Germany

[3] Univ Hosp Freiburg, Dept Nucl Med, D-79106 Freiburg, Germany

[4] Res Ctr Juelich, Inst Neurosci & Med, D-52425 Julich, Germany

来源：

NUCLEAR MEDICINE AND BIOLOGY | 2012年 / 39卷 / 07期

关键词：

Positron emission tomography; F-18]desmethoxyfallypride; Neurodegeneration; Huntington's disease; Parkinson's disease; Animal model; ANIMAL-MODELS; PARKINSONS; BINDING; LESIONS; BRAIN;

D O I：

10.1016/j.nucmedbio.2012.04.003

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

Introduction: [F-18]desmethoxyfallypride ([F-18]DMFP) is a promising tracer for longitudinal assessment of striatal dopamine D2/D3-receptor (D2R) availability by positron emission tomography (PET) in small animal models. We explored the feasibility of [F-18]DMFP-PET to image D2R availability in rat models of Huntington's (HD) and Parkinson's disease (PD). Methods: Animals received either unilateral intrastriatal quinolinic acid lesions or medial forebrain bundle injections of 6-OHDA to produce the loss of striatal projection neurones or deplete the striatal dopamine, corresponding to established animal models for HD and PD, respectively. Three weeks after lesioning, PET scans were acquired on a microPET Focus 120 system following the tail vein injection of [F-18]DMFP. Results: [F-18]DMFP-PET clearly visualized lesion induced decreases and increases of D2R availability. In vivo estimates of D2R binding and changes thereof gained by pharmacokinetic analyses correlated significantly with D2R density and its change provided by in vitro [H-3]raclopride-autoradiography. Conclusions: In conclusion, [F-18]DMFP-PET is a suitable method for in vivo D2R-assessment in preclinical research, e.g for monitoring cell-based therapies. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：1077 / 1080

页数：4

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