Spotted Fever Group Rickettsia in Yunnan Province, China

被引：15
作者：
Liang, Chang-wei [1 ,2 ]
Zhao, Jing-bo [2 ]
Li, Juan [3 ]
Chang, Li-tao [3 ]
Yu, Hui-lan [1 ]
Zhang, Li-xia [1 ]
Zhang, Li-juan [1 ]
Yu, Xue-jie [4 ]
机构：
[1] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Beijing, Peoples R China
[2] Harbin Med Univ, Sch Publ Hlth, Harbin, Peoples R China
[3] Yunnan Prov Ctr Dis Control & Prevent, Kunming, Peoples R China
[4] Univ Texas Med Branch, Dept Pathol, Ctr Biodef & Emerging Infect Dis, Seally Ctr Vaccine Dev, Galveston, TX USA
来源：
VECTOR-BORNE AND ZOONOTIC DISEASES | 2012年 / 12卷 / 04期
关键词：
Bacterial load; Prevalence; Real-time PCR; Spotted fever group rickettsiae; TIME PCR ASSAYS; PHYLOGENETIC ANALYSIS; PROTEIN ROMPA; GENE; ANTIBODIES; DIFFERENTIATION; IDENTIFICATION; TYPHUS; DOGS; HEILONGJIANGENSIS;
D O I：
10.1089/vbz.2011.0835
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
Information about spotted fever group (SFG) rickettsiae in southern China remains sparse. A specific and sensitive real-time PCR assay for detection of SFG rickettsiae was established and used to detect the prevalence rate of SFG rickettsiae in Yunnan Province, China. The limit of detection (LOD) of our real-time PCR was 200 copies per reaction, which is more sensitive than the previously developed nested PCR assays for Rickettsia. We tested 265 blood samples (127 goats, 78 dogs, and 60 cattle) collected from Yunnan Province using the real-time PCR assay and revealed that the prevalence of SFG rickettsiae among dogs, cattle, and goats were 14.10%, 23.33%, and 24.41%, respectively. The SFG rickettsiae detected in animals in Yunnan Province were classified into two genotypes: a unique group that is different from all known SFG rickettsiae species, and R. heilongjiangensis.
引用
收藏
页码：281 / 286
页数：6
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