A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

被引：36

作者：

Elwin, K. ^{[1
]}

Robinson, G. ^{[1
]}

Hadfield, S. J. ^{[1
]}

Fairclough, H. V. ^{[2
]}

Iturriza-Gomara, M. ^{[3
]}

Chalmers, R. M. ^{[1
]}

机构：

[1] Singleton Hosp, UK Cryptosporidium Reference Unit, Swansea SA2 8QA, W Glam, Wales

[2] Aberystwyth Univ, Inst Biol Environm & Rural Sci, Aberystwyth SY23 3DA, Ceredigion, Wales

[3] Hlth Protect Agcy, Virus Reference Dept, MS Colindale, London NW9 5EQ, England

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 89卷 / 01期

关键词：

Cryptosporidium; DNA extraction; Real time PCR; FECAL SAMPLES; RAPID METHOD; HUMAN FECES; PARVUM; SPECIFICITY; SENSITIVITY; DIAGNOSIS; OOCYSTS;

D O I：

10.1016/j.mimet.2012.02.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P = 0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：38 / 40

页数：3

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