DARS-AS1 modulates cell proliferation and migration of gastric cancer cells by regulating miR-330-3p/NAT10 axis

被引：8
作者：
Du, Chunjuan [1 ,2 ]
Han, Xia [2 ]
Zhang, Yanyan [3 ]
Guo, Fengli [4 ]
Yuan, Haibin [5 ]
Wang, Feng [2 ]
Li, Mianli [2 ]
Ning, Fangling [2 ]
Wang, Weibo [1 ]
机构：
[1] Shandong Univ, Shandong Prov Hosp, Dept Oncol, 324 Jingwuweiqi Rd, Jinan 250021, Shandong, Peoples R China
[2] Binzhou Med Univ Hosp, Dept Oncol, Binzhou 256603, Shandong, Peoples R China
[3] Binzhou Med Univ Hosp, Dept Pediat, Binzhou 256603, Shandong, Peoples R China
[4] Binzhou Med Univ Hosp, Dept Breast Surg, Binzhou 256603, Shandong, Peoples R China
[5] Binzhou Med Univ Hosp, Dept Hlth Management, Binzhou 256603, Shandong, Peoples R China
来源：
OPEN MEDICINE | 2022年 / 17卷 / 01期
关键词：
gastric cancer; DARS-AS1; NAT10; miR-330-3p; progression; LONG NONCODING RNA; OVARIAN-CANCER; LNCRNA; PROGRESSION; SUPPRESSES; PROTEIN; NAT10;
D O I：
10.1515/med-2022-0583
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The long noncoding RNA DARS-AS1 was aberrantly expressed and participated in several human cancer progressions, whereas whether DARS-AS1 is involved in human gastric cancer remains unclear. This study aimed to investigate the influence of DARS-AS1 on gastric cancer progression and explore the potential regulatory network of DARS-AS1/miR-330-3p/NAT10. The expression levels of DARS-AS1, miR-330-3p, and NAT10 were measured by quantitative real-time polymerase chain reaction. The CCK-8 assay and Transwell assay were used to determine the cell viability, migration, and invasion capacities, respectively. The target association between miR-330-3p and DARS-AS1 or NAT10 was confirmed using a luciferase reporter assay. In result, DARS-AS1 levels were elevated in tumor tissues and associated with shorter overall survival in patients with gastric cancer. Knockdown of DARS-AS1 could hamper cell viability, migration, and invasion in gastric cancer cells. DARS-AS1 acts as a competitive endogenous RNA to regulate the NAT10 expression by sponging miR-330-3p in gastric cancer cells. In conclusion, DARS-AS1 was elevated in gastric cancer, and DARS-AS1/miR-330-3p/NAT10 signaling offered some new horizons for predicting prognosis and a novel therapeutic method for the treatment of gastric cancer.
引用
收藏
页码：2036 / 2045
页数：10
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