Gallic acid potentiates the apoptotic effect of paclitaxel and carboplatin via overexpression of Bax and P53 on the MCF-7 human breast cancer cell line

被引:74
|
作者
Aborehab, Nora M. [1 ]
Elnagar, Mohamed R. [2 ]
Waly, Nermien E. [3 ,4 ]
机构
[1] October Univ Modern Sci & Arts MSA, Dept Biochem, Fac Pharm, Giza 12451, Egypt
[2] Al Azhar Univ, Dept Pharmacol & Toxicol, Fac Pharm, Cairo, Egypt
[3] Helwan Univ, Dept Physiol, Fac Med, Cairo, Egypt
[4] Creighton Sch Med, Dept Med Educ, Omaha, NE USA
关键词
Bax; carboplatin; gallic acid; MCF-7; P53; paclitaxel; PHASE-II TRIAL; TRASTUZUMAB; GROWTH; PROLIFERATION; ACTIVATION; CISPLATIN; THERAPY; WOMEN;
D O I
10.1002/jbt.22638
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Despite advances in treatment, breast cancer remains the widest spread disease among females with a high mortality rate. We investigated the potential effects of gallic acid (GA) as supportive therapy in the management of breast cancer. Anti-cancer activity with GA alone or in combination with paclitaxel and/or carboplatin was assessed by MTT assay and flow cytometry using annexin V/propidium iodide. The mechanism underlying the antiproliferative effects was investigated by measuring the expression of the pro-apoptotic marker (Bax), CASP-3, anti-apoptotic (Bcl-2), and, tumor suppressor (p53) by real-time polymerase chain reaction (RT-PCR) and western blot analysis. Cell cycle analysis was performed for the MCF-7 breast cancer cell line. GA, paclitaxel, and carboplatin alone or in combination arrested cell cycle progression at the G2/M phase and induced Pre-G1 apoptosis. RT-PCR showed that the triplet combination significantly raised P53, Bax, and CASP-3 mRNA expression (20.1 +/- 1.41, 16.6 +/- 0.43, and 20.04 +/- 1.61, respectively) in MCF-7 cells when compared to single or combined treatment (p < .0001) while anti-apoptotic Bcl-2 mRNA levels were decreased in all treated groups compared to untreated cells. Western blot data of tested apoptotic factors were consistent with RT-PCR results. For the first time, we show that a minimum non-toxic concentration of GA increased the efficacy of paclitaxel- and carboplatin-induced MCF-7 apoptotic cell death.
引用
收藏
页数:11
相关论文
共 50 条
  • [21] Cytotoxic and Apoptotic Effects of Vanadyl Sulfate on MCF-7 Breast Cancer Cell Line
    Dehdashti, Maryam
    Abbasy, Zahra
    Arani, Hamid Zaferani
    Atashi, Hesam Adin
    Salimi-Tabatabaee, Seyed Alireza
    Ghasemi, Afsaneh
    Fereidouni, Zhila
    Marzouni, Hadi Zare
    Zakeri, Habib
    Mirmalek, Seyed Abbas
    GALEN MEDICAL JOURNAL, 2023, 12
  • [22] Methionine cytotoxicity in the human breast cancer cell line MCF-7
    Kim, HH
    Park, CS
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2003, 39 (3-4) : 117 - 119
  • [23] Weightlessness acts on human breast cancer cell line MCF-7
    Vassy, J
    Portet, S
    Beil, M
    Millet, G
    Fauvel-Lafève, F
    Gasset, G
    Schoevaert, D
    SPACE LIFE SCIENCES: GRAVITATIONAL BIOLOGY: 2002, 2003, 32 (08): : 1595 - 1603
  • [24] Twist overexpression promotes chromosomal instability in the breast cancer cell line MCF-7
    Vesuna, Farhad
    Winnard, Paul, Jr.
    Raman, Venu
    Glackin, Carlotta
    CANCER GENETICS AND CYTOGENETICS, 2006, 167 (02) : 189 - 191
  • [25] Antiproliferative effect of dexamethasone in the MCF-7 breast cancer cell line
    Buxant, Frederic
    Kindt, Nadege
    Laurent, Guy
    Noel, Jean-Christophe
    Saussez, Sven
    MOLECULAR MEDICINE REPORTS, 2015, 12 (03) : 4051 - 4054
  • [26] A comparison of the effects of tributyltin chloride and triphenyltin chloride on cell proliferation, proapoptotic p53, Bax, and antiapoptotic Bcl-2 protein levels in human breast cancer MCF-7 cell line
    Fickova, Maria
    Macho, Ladislav
    Brtko, Julius
    TOXICOLOGY IN VITRO, 2015, 29 (04) : 727 - 731
  • [27] HDAC3-ERα Selectively Regulates TNF-α-Induced Apoptotic Cell Death in MCF-7 Human Breast Cancer Cells via the p53 Signaling Pathway
    Park, Seung-Ho
    Kim, Hyunhee
    Kwak, Sungmin
    Jeong, Ji-Hoon
    Lee, Jangho
    Hwang, Jin-Taek
    Choi, Hyo-Kyoung
    Choi, Kyung-Chul
    CELLS, 2020, 9 (05)
  • [28] Overexpression of bax sensitizes human breast cancer MCF-7 cells to radiation-induced apoptosis
    Sakakura, C
    Sweeney, EA
    Shirahama, T
    Igarashi, Y
    Hakomori, S
    Nakatani, H
    Tsujimoto, H
    Imanishi, T
    Ohgaki, M
    Ohyama, T
    Yamazaki, J
    Hagiwara, A
    Yamaguchi, T
    Sawai, K
    Takahashi, T
    INTERNATIONAL JOURNAL OF CANCER, 1996, 67 (01) : 101 - 105
  • [29] Synergistic effect of hyperforin and paclitaxel on growth inhibition, apoptotic mediator activation in MCF-7 human breast cancer cells
    Barathan, Muttiah
    Zulpa, Ahmad Khusairy
    Hoong, See Mee
    Vellasamy, Kumutha Malar
    Vadivelu, Jamuna
    JOURNAL OF TAIBAH UNIVERSITY FOR SCIENCE, 2021, 15 (01): : 918 - 927
  • [30] Antioxidant and Apoptotic Effects of an Aqueous Extract of Urtica dioica on the MCF-7 Human Breast Cancer Cell Line
    Fattahi, Sadegh
    Ardekani, Ali Motevalizadeh
    Zabihi, Ebrahim
    Abedian, Zeinab
    Mostafazadeh, Amrollah
    Pourbagher, Roghayeh
    Akhavan-Niaki, Haleh
    ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 2013, 14 (09) : 5317 - 5323