Different extenders in the cryopreservation of bovine epididymal spermatozoa

被引：16

作者：

Papa, Patricia M. ^{[1
]}

Papa, Frederico O. ^{[1
]}

Oliveira, Leticia A. ^{[2
]}

Guasti, Priscilla N. ^{[1
]}

Castilho, Calie ^{[2
]}

Giometti, Ines Cristina ^{[2
]}

机构：

[1] UNESP, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, BR-18610970 Botucatu, SP, Brazil

[2] Oeste Paulista Univ, UNOESTE, BR-19067175 Presidente Prudente, SP, Brazil

来源：

ANIMAL REPRODUCTION SCIENCE | 2015年 / 161卷

基金：

巴西圣保罗研究基金会;

关键词：

Sperm Talp; Semen extender; Epididymal spermatozoa; Bull; Cryopreservation; IN-VITRO FERTILIZATION; SPERM CAPACITATION; STALLION SPERMATOZOA; SEMEN QUALITY; FERTILITY; STORAGE; PHOSPHORYLATION; OSMOLALITY; ALBUMIN; VOLUME;

D O I：

10.1016/j.anireprosci.2015.08.004

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The objective of this study was to evaluate the effects of two different egg yolk extenders incubated with or without Sperm Talp on the motility and plasma membrane integrity of cryopreserved bovine epididymal spermatozoa after freezing. Twenty-five testicles with epididymides from mature bulls were collected at the abattoir. Epididymal sperm recovery was performed by retrograde flushing using a skim milk-extender (Botu-Semen (TM)). After recovery, sperm were incubated either without or with Sperm Talp and then submitted to centrifugation. For the freezing process, half of the testes were processed with Tris egg yolk extender, and half were processed with Botu-Bov (TM) egg yolk extender. Samples incubated in Sperm Talp exhibited better results than epididymal spermatozoa that were incubated without Sperm Talp (p<0.05). Both Botu-Bov (TM) and Tris could be utilised to freeze sperm from the bovine epididymides if the sperm were previously incubated with Sperm Talp. The extenders examined in this work did not differ in their effect on plasma membrane integrity after freezing. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：58 / 63

页数：6

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