Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage

被引：99

作者：

Sjovall, Fredrik ^{[1
,2
]}

Ehinger, Johannes K. H. ^{[1
]}

Marelsson, Sigurdur E. ^{[1
,3
]}

Morota, Saori ^{[1
]}

Frostner, Eleonor Asander ^{[1
]}

Uchino, Hiroyuki ^{[4
]}

Lundgren, Johan ^{[3
]}

Arnbjornsson, Einar ^{[5
]}

Hansson, Magnus J. ^{[1
,6
]}

Fellman, Vineta ^{[3
,7
]}

Elmer, Eskil ^{[1
,8
]}

机构：

[1] Lund Univ, Mitochondrial Pathophysiol Unit, S-22184 Lund, Sweden

[2] Univ Copenhagen, Rigshosp, Intens Care Unit 4131, DK-2100 Copenhagen, Denmark

[3] Lund Univ, Skane Univ Hosp, Dept Pediat, S-22185 Lund, Sweden

[4] Tokyo Med Univ, Dept Anesthesiol, Tokyo 1608402, Japan

[5] Lund Univ, Skane Univ Hosp, Dept Pediat Surg, S-22185 Lund, Sweden

[6] Lund Univ, Skane Univ Hosp, Dept Clin Physiol, S-22185 Lund, Sweden

[7] Univ Helsinki, Childrens Hosp, Helsinki Univ Hosp, FIN-00029 Helsinki, Finland

[8] Lund Univ, Skane Univ Hosp, Dept Clin Neurophysiol, S-22185 Lund, Sweden

来源：

MITOCHONDRION | 2013年 / 13卷 / 01期

基金：

瑞典研究理事会;

关键词：

Human; Platelets; Mitochondria; OXPHOS; High-resolution respirometry; Mitochondrial disorders; SKELETAL-MUSCLE; OXIDATIVE-PHOSPHORYLATION; DYSFUNCTION; METABOLISM; DISEASE; SENESCENCE; DEFECTS; TISSUE;

D O I：

10.1016/j.mito.2012.11.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondria] physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondria] respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability. (c) 2012 Elsevier B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：7 / 14

页数：8

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