Hyperhomocysteinemia induces injury in olfactory bulb neurons by downregulating Hes1 and Hes5 expression

被引：3

作者：

Zhang, Jing-wen ^{[1
,2
]}

Pang, Bo ^{[1
]}

Zhao, Qi ^{[1
]}

Chang, Yue ^{[1
]}

Wang, Yi-li ^{[2
]}

Jiang, Yi-deng ^{[1
]}

Jing, Li ^{[1
]}

机构：

[1] Ningxia Med Univ, Sch Basic Med Sci, Ningxia Key Lab Cerebrocranial Dis Incubat Base, Natl Key Lab, Yinchuan, Ningxia Hui Aut, Peoples R China

[2] Xi An Jiao Tong Univ, Inst Immunopathol, Med Sch, Key Lab Biomed Informat Engn,Minist Educ, Xian, Shaanxi, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2018年 / 13卷 / 02期

基金：

中国国家自然科学基金;

关键词：

nerve regeneration; olfactory bulb; apoptosis; neurons; Nissl body; homocysteine; hairy enhancer of split 1; hairy enhancer of split 5; neural regeneration; ALZHEIMERS-DISEASE; CARDIOVASCULAR-DISEASE; OXIDATIVE STRESS; HOMOCYSTEINE; CELLS; RATS; MICE; BRAIN; ATHEROSCLEROSIS; TRANSCRIPTION;

D O I：

10.4103/1673-5374.220779

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Hyperhomocysteinemia has been shown to be associated with neurodegenerative diseases; however, lesions or histological changes and mechanisms underlying homocysteine-induced injury in olfactory bulb neurons remain unclear. In this study, hyperhomocysteinemia was induced in apolipoprotein E-deficient mice with 1.7% methionine. Pathological changes in the olfactory bulb were observed through hematoxylin-eosin and Pischingert staining. Cell apoptosis in the olfactory bulb was determined through terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining. Transmission electron microscopy revealed an abnormal ultrastructure of neurons. Furthermore, immunoreactivity and expression of the hairy enhancer of the split 1 (Hes1) and Hes5 were measured using immunohistochemistry, immunofluorescence, and western blot assay. Our results revealed no significant structural abnormality in the olfactory bulb of hyperhomocysteinemic mice. However, the number of TUNEL-positive cells increased in the olfactory bulb, lipofuscin and vacuolization were visible in mitochondria, and the expression of Hes1 and Hes5 decreased. These findings confirm that hyperhomocysteinemia induces injury in olfactory bulb neurons by downregulating Hes1 and Hes5 expression.

引用

页码：272 / 279

页数：8

共 33 条

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