The invasive Asian bush mosquito Aedes japonicus found in the Netherlands can experimentally transmit Zika virus and Usutu virus

被引：30

作者：

Abbo, Sandra R. ^{[1
]}

Visser, Tessa M. ^{[2
]}

Wang, Haidong ^{[1
]}

Goertz, Giel P. ^{[1
]}

Fros, Jelke J. ^{[1
]}

Abma-Henkens, Marleen H. C. ^{[1
]}

Geertsema, Corinne ^{[1
]}

Vogels, Chantal B. F. ^{[6
]}

Koopmans, Marion P. G. ^{[3
]}

Reusken, Chantal B. E. M. ^{[3
,6
,7
]}

Hall-Mendelin, Sonja ^{[4
]}

Hall, Roy A. ^{[5
]}

van Oers, Monique M. ^{[1
]}

Koenraadt, Constantianus J. M. ^{[2
]}

Pijlman, Gorben P. ^{[1
]}

机构：

[1] Wageningen Univ & Res, Virol Lab, Wageningen, Netherlands

[2] Wageningen Univ & Res, Entomol Lab, Wageningen, Netherlands

[3] Erasmus MC, Dept Virosci, Rotterdam, Netherlands

[4] Dept Hlth, Publ Hlth Virol Forens & Sci Serv, Coopers Plains, Australia

[5] Univ Queensland, Sch Chem & Mol Biosci, St Lucia, Qld, Australia

[6] Yale Sch Publ Hlth, Epidemiol Microbial Dis, New Haven, CT USA

[7] Natl Inst Publ Hlth & Environm, Ctr Infect Dis Control, Bilthoven, Netherlands

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2020年 / 14卷 / 04期

基金：

欧盟地平线“2020”;

关键词：

ENCEPHALITIS-VIRUS; SMALL RNAS; FLAVIVIRUS; COMPETENCE; INFECTION;

D O I：

10.1371/journal.pntd.0008217

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background The Asian bush mosquito Aedes japonicus is invading Europe and was first discovered in Lelystad, the Netherlands in 2013, where it has established a permanent population. In this study, we investigated the vector competence of Ae. japonicus from the Netherlands for the emerging Zika virus (ZIKV) and zoonotic Usutu virus (USUV). ZIKV causes severe congenital microcephaly and Guillain-Barre syndrome in humans. USUV is closely related to West Nile virus, has recently spread throughout Europe and is causing mass mortality of birds. USUV infection in humans can result in clinical manifestations ranging from mild disease to severe neurological impairments. Methodology/Principal findings In our study, field-collected Ae. japonicus females received an infectious blood meal with ZIKV or USUV by droplet feeding. After 14 days at 28 degrees C, 3% of the ZIKV-blood fed mosquitoes and 13% of the USUV-blood fed mosquitoes showed virus-positive saliva, indicating that Ae. japonicus can transmit both viruses. To investigate the effect of the mosquito midgut barrier on virus transmission, female mosquitoes were intrathoracically injected with ZIKV or USUV. Of the injected mosquitoes, 96% (ZIKV) and 88% (USUV) showed virus-positive saliva after 14 days at 28 degrees C. This indicates that ZIKV and USUV can efficiently replicate in Ae. japonicus but that a strong midgut barrier is normally restricting virus dissemination. Small RNA deep sequencing of orally infected mosquitoes confirmed active replication of ZIKV and USUV, as demonstrated by potent small interfering RNA responses against both viruses. Additionally, de novo small RNA assembly revealed the presence of a novel narna-virus in Ae. japonicus. Conclusions/Significance Given that Ae. japonicus can experimentally transmit arthropod-borne viruses (arboviruses) like ZIKV and USUV and is currently expanding its territories, we should consider this mosquito as a potential vector for arboviral diseases in Europe. Author summary Arthropod-borne viruses (arboviruses) cause a high disease burden in humans and animals. Zika virus (ZIKV) causes microcephaly and Guillain-Barre syndrome in humans, whereas Usutu virus (USUV) induces high mortality in birds and neurological disease in humans. The spread of arboviruses such as ZIKV and USUV is determined by the presence of mosquitoes that can transmit these viruses from one vertebrate host to the next. Here, we investigate the risk of transmission of ZIKV and USUV by the Asian bush mosquito Aedes japonicus. This mosquito is invading Europe and is currently present in the Netherlands. We found that field-collected Ae. japonicus mosquitoes can experimentally transmit ZIKV and USUV. Of the orally infected mosquitoes, 3% (ZIKV) and 13% (USUV) showed virus-positive saliva after 14 days at 28 degrees C. We also found that ZIKV and USUV activated the antiviral RNA interference immune response of Ae. japonicus. Moreover, a strong barrier in the mosquito midgut restricted virus dissemination, since 96% (ZIKV) and 88% (USUV) of the mosquitoes injected with ZIKV or USUV showed virus-positive saliva. Additionally, we discovered a narnavirus in Ae. japonicus. Given that Ae. japonicus can transmit ZIKV and USUV, we should consider this mosquito as a potential vector for arboviral diseases in Europe.

引用

页码：1 / 22

页数：22

共 55 条

[31] The 25-26 nt Small RNAs in Phytophthora parasitica Are Associated with Efficient Silencing of Homologous Endogenous Genes [J].

Jia, Jinbu ;

Lu, Wenqin ;

Zhong, Chengcheng ;

Zhou, Ran ;

Xu, Junjie ;

Liu, Wei ;

Gou, Xiuhong ;

Wang, Qinhu ;

Yin, Junliang ;

Xu, Cheng ;

Shan, Weixing .

FRONTIERS IN MICROBIOLOGY, 2017, 8

[32] Out of the bush: the Asian bush mosquito Aedes japonicus japonicus (Theobald, 1901) (Diptera, Culicidae) becomes invasive [J].

Kampen, Helge ;

Werner, Doreen .

PARASITES & VECTORS, 2014, 7

[33]

Langmead B, 2012, NAT METHODS, V9, P357, DOI [10.1038/nmeth.1923, 10.1038/NMETH.1923]

[34] Mosquitoes of North-Western Europe as Potential Vectors of Arboviruses: A Review [J].

Martinet, Jean-Philippe ;

Ferte, Hubert ;

Failloux, Anna-Bella ;

Schaffner, Francis ;

Depaquit, Jerome .

VIRUSES-BASEL, 2019, 11 (11)

[35] Zika Virus Pathogenesis and Tissue Tropism [J].

Miner, Jonathan J. ;

Diamond, Michael S. .

CELL HOST & MICROBE, 2017, 21 (02) :134-142

[36] Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma [J].

Morazzani, Elaine M. ;

Wiley, Michael R. ;

Murreddu, Marta G. ;

Adelman, Zach N. ;

Myles, Kevin M. .

PLOS PATHOGENS, 2012, 8 (01)

[37] Zika Virus [J].

Musso, Didier ;

Gubler, Duane J. .

CLINICAL MICROBIOLOGY REVIEWS, 2016, 29 (03) :487-524

[38] Viral RNA Intermediates as Targets for Detection and Discovery of Novel and Emerging Mosquito-Borne Viruses [J].

O'Brien, Caitlin A. ;

Hobson-Peters, Jody ;

Yam, Alice Wei Yee ;

Colmant, Agathe M. G. ;

McLean, Breeanna J. ;

Prow, Natalie A. ;

Watterson, Daniel ;

Hall-Mendelin, Sonja ;

Warrilow, David ;

Ng, Mah-Lee ;

Khromykh, Alexander A. ;

Hall, Roy A. .

PLOS NEGLECTED TROPICAL DISEASES, 2015, 9 (03)

[39]

REED L. J., 1938, AMER JOUR HYG, V27, P493

[40] Widespread Usutu virus outbreak in birds in the Netherlands, 2016 [J].

Rijks, J. ;

Kik, M. ;

Slaterus, R. ;

Foppen, R. ;

Stroo, A. ;

Ijzer, J. ;

Stahl, J. ;

Grone, A. ;

Koopmans, M. ;

van der Jeugd, H. ;

Reusken, C. .

EUROSURVEILLANCE, 2016, 21 (45) :2-7

← 1 2 3 4 5 6 →