Comparison of promidium monoazide and Reagent D for discrimination of viableAphelenchoides besseyiusing real-time PCR

被引：1

作者：

Sert Celik, Elvan ^{[1
,2
]}

Ozalp, Tevfik ^{[1
]}

Devran, Zubeyir ^{[1
]}

机构：

[1] Univ Akdeniz, Fac Agr, Dept Plant Protect, Antalya, Turkey

[2] Argeto Co, Antalya, Turkey

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2020年 / 158卷 / 03期

关键词：

Aphelenchoides besseyi; Viability; Propidium monoazide; Reagent D; TaqMan probe; PROPIDIUM MONOAZIDE; APHELENCHOIDES-BESSEYI; LISTERIA-MONOCYTOGENES; QUANTIFICATION; COLI;

D O I：

10.1007/s10658-020-02090-x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Aphelenchoides besseyiChristie, 1942 is a seed-borne nematode and causes economic yield losses in rice. Information on the viability ofA. besseyiin stored rice seeds is important to manage this nematode. In this study, propidium monoazide (PMA) and Reagent D, combined with qPCR, were used for discrimination of viableA. besseyiindividuals.A. besseyiindividuals that were live, stored for 1 year or heat-treated (40 or 60 degrees C, 15 min) were exposed to PMA and Reagent-D dyes. In addition,A. besseyiindividuals not treated with any chemicals were used as a control. DNA was then isolated from all individuals and real-time PCR was carried out. Ct values for samples treated with Reagent-D and PMA were different from untreated samples. Results showed that live individuals of the rice white tipe nematode could be distinguished from non-viable individuals using Reagent-D or PMA combined with qPCR.

引用

页码：767 / 771

页数：5

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