Histone acetyltransferase-1 regulates integrity of cytosolic histone H3-H4 containing complex

被引：32

作者：

Barman, Hirak Kumar ^{[1
]}

Takami, Yasunari ^{[1
]}

Nishijima, Hitoshi ^{[3
]}

Shibahara, Kei-ichi ^{[3
]}

Sanematsu, Fumiyuki ^{[1
]}

Nakayama, Tatsuo ^{[1
,2
]}

机构：

[1] Miyazaki Univ, Dept Med Sci, Sect Biochem & Mol Biol, Miyazaki 8891692, Japan

[2] Miyazaki Univ, Dept Life Sci, Frontier Sci Res Ctr, Miyazaki 8891692, Japan

[3] Natl Inst Genet, Dept Integrated Genet, Mishima, Shizuoka 4118540, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 373卷 / 04期

关键词：

chromatin; histone chaperone; histone acetyltransferase;

D O I：

10.1016/j.bbrc.2008.06.100

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Amounts of soluble histones in cells are tightly regulated to ensure supplying them for the newly synthesized DNA and preventing the toxic effect of excess histones. Prior to incorporation into chromatin, newly synthesized histones H3 and H4 are highly acetylated in pre-deposition complex, wherein H4 is di-acetylated at Lys-5 and Lys-12 residues by histone acetyltransferase-1 (Hat1), but their role in histone metabolism is still unclear. Here, using chicken DT 40 cytosolic extracts, we found that histones H3/H4 and their chaperone Asf1, including RbAp48, a regulatory subunit of Hat1 enzyme, were associated with Hat1. Interestingly, in HAT1-deficient cells, cytosolic histones H3/H4 fractions on sucrose gradient centrifugation, having a sedimentation coefficient of 5-6S in DT40 cells, were shifted to lower molecular mass fractions, with Asf1. Further, sucrose gradient fractionation of semi-purified tagged Asf1-complexes showed the presence of Hat1, RbAp48 and histones H3/H4 at 5-6S fractions in the complexes. These findings suggest the possible involvement of Hat1 in regulating cytosolic H3/H4 pool mediated by Asf1-containing cytosolic H3/H4 pre-deposition complex. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：624 / 630

页数：7

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