Tomato yellow leaf curl Thailand virus-[Y72] from Yunnan is a monopartite begomovirus associated with DNAβ

被引：33
作者：
Guo, Wei [1 ]
Yang, Xiuling [1 ]
Xie, Yan [1 ]
Cui, Xiaofeng [1 ]
Zhou, Xueping [1 ]
机构：
[1] Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310029, Zhejiang, Peoples R China
来源：
VIRUS GENES | 2009年 / 38卷 / 02期
关键词：
Tomato yellow leaf curl Thailand virus; Monopartite begomovirus; Evolution; GEMINIVIRUS DISEASE COMPLEXES; CASSAVA MOSAIC-VIRUS; MOLECULAR CHARACTERIZATION; INFECTING BEGOMOVIRUSES; INDIA; AGROINOCULATION; IDENTIFICATION; POLYMERASE; COMPONENT; THREAT;
D O I：
10.1007/s11262-009-0327-4
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Previous studies have shown that isolates of tomato yellow leaf curl Thailand virus (TYLCTHV) originated from Thailand are bipartite begomoviruses, while all the seven TYLCTHV isolates found in China are associated with DNA beta molecules. In this study, infectious clones of TYLCTHV isolate Y72 (TYLCTHV-[Y72]) and its DNA beta were constructed to verify the bipartite or monopartite nature of TYLCTHV. Agroinoculation showed that TYLCTHV-[Y72] alone was able to induce significant symptoms in Nicotiana benthamiana, Nicotiana glutinosa, and Solanum lycopersicum plants, but co-inoculation with its associated satellite DNA beta produced more severe symptoms, which is similar to tobacco curly shoot virus. Southern blot results showed that TYLCTHV DNA beta could increase the virus accumulation in systemically infected tissues. Thus, TYLCTHV-[Y72] is a monopartite begomovirus, which may represent an evolutionary intermediate between the begomoviruses requiring DNA beta and begomoviruses dispensable of DNA beta.
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页码：328 / 333
页数：6
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