Effects of diazinon on acetylcholinesterase activity and lipid peroxidation in the brain of Oreochromis niloticus

被引：121

作者：

Üner, N ^{[1
]}

Oruç, EÖ ^{[1
]}

Sevgiler, Y ^{[1
]}

Sahin, N ^{[1
]}

Durmaz, H ^{[1
]}

Usta, D ^{[1
]}

机构：

[1] Cukurova Univ, Fac Sci & Letters, Dept Biol, TR-01330 Adana, Turkey

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2006年 / 21卷 / 03期

关键词：

diazinon; fish; Oreochromis niloticus; brain; acetylcholinesterase; lipid peroxidation;

D O I：

10.1016/j.etap.2005.08.007

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The aim of this study was to investigate the effects of organophosphorus (OP) pesticide diazinon on acetycholinesterase (AChE: EC 3.1.1.7) activity and its relationship to lipid peroxidation (LPO) in the brain of a freshwater fish, Oreochromis niloticus. Malondialdehyde (MDA) content was used as biomarker for LPO. Fish were exposed to 1 and 2 mg/L sublethal concentrations of diazinon for 1, 7, 15 and, 30 days. In the entire experimental group, AChE activity in brain significantly decreased (up to 93% of control), whereas MDA content decreased after 1 day, and increased after 7 and 15 days of exposures. MDA was in similar level with the control group after diazinon exposure of 30 days. The findings of the present study show that diazinon inhibited AChE activity and it has LPO-inducing potential in fish. The inhibition of AChE activity in the brain of O. niloticus correlated with increased MDA levels after 7 and 15 days diazinon exposures (r= -0.66 1, P < 0.0 19; r = -0.652, P < 0.022, respectively). (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：241 / 245

页数：5

共 41 条

[1] MALATHION TOXICITY - INVIVO INHIBITION OF ACETYLCHOLINESTERASE IN THE FISH BRACHYDANIO-RERIO (CYPRINIDAE) [J].

ANSARI, BA ;

KUMAR, K .

TOXICOLOGY LETTERS, 1984, 20 (03) :283-287

[2] DIAZINON TOXICITY - ACTIVITIES OF ACETYLCHOLINESTERASE AND PHOSPHATASES IN THE NERVOUS-TISSUE OF ACETYLCHOLINESTERASE AND PHOSPHATASES IN THE NERVOUS-TISSUE OF ZEBRA FISH, BRACHYDANIO-RERIO (CYPRINIDAE) [J].

ANSARI, BA ;

ASLAM, M ;

KUMAR, K .

ACTA HYDROCHIMICA ET HYDROBIOLOGICA, 1987, 15 (03) :301-306

[3]

*APHA AWWA WPCF, 1981, STAND METH CLIN CHEM, P616

[4] BIOCHEMICAL AND SUBCELLULAR CHANGES IN CARP EXPOSED TO THE ORGANOPHOSPHORUS METHIDATHION AND THE PYRETHROID DELTAMETHRIN [J].

BALINT, T ;

SZEGLETES, T ;

SZEGLETES, Z ;

HALASY, K ;

NEMCSOK, J .

AQUATIC TOXICOLOGY, 1995, 33 (3-4) :279-295

[5] Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers [J].

Banerjee, BD ;

Seth, V ;

Bhattacharya, A ;

Pasha, ST ;

Chakraborty, AK .

TOXICOLOGY LETTERS, 1999, 107 (1-3) :33-47

[6] Antioxidant defenses and lipid peroxidation in the cerebral cortex and hippocampus following acute exposure to malathion and/or zinc chloride [J].

Brocardo, PS ;

Pandolfo, P ;

Takahashi, RN ;

Rodrigues, ALS ;

Dafre, AL .

TOXICOLOGY, 2005, 207 (02) :283-291

[7]

BRZEZINSKI J, 1973, POL J PHARMACOL PHAR, V25, P313

[8] The interplay of glutathione-related processes in antioxidant defense [J].

Cnubben, NHP ;

Rietjens, IMCM ;

Wortelboer, H ;

van Zanden, J ;

van Bladeren, PJ .

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2001, 10 (04) :141-152

[9]

Corbett J, 1974, BIOCH MODE ACTION PE

[10] Concentration effects of selected insecticides on brain acetylcholinesterase in the common carp (Cyprinus carpio L.) [J].

Dembélé, K ;

Haubruge, E ;

Gaspar, C .

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2000, 45 (01) :49-54

← 1 2 3 4 5 →