Use of urea-polyacrylamide electrophoresis for discrimination of A1 and A2 beta casein variants in raw cow's milk

被引：22

作者：

Angel Duarte-Vazquez, Miguel ^{[1
]}

Raul Garcia-Ugalde, Carlos ^{[1
]}

Erika Alvarez, Blanca ^{[2
]}

Mariana Villegas, Laura ^{[1
]}

Estela Garcia-Almendarez, Blanca ^{[2
]}

Luis Rosado, Jorge ^{[3
]}

Regalado, Carlos ^{[2
]}

机构：

[1] Ctr Invest & Desarrollo Tecnol Enfermedades Cron, Ave Jur 122,Parque Ind Queretaro, Queretaro 76220, Qro, Mexico

[2] Univ Autonoma Queretaro CU, Fac Quim, DIPA, Cerro Campanas S-N, Queretaro 76010, Qro, Mexico

[3] Univ Autonoma Queretaro Campus Juriquilla, Fac Ciencias Nat, Ave Ciencias S-N, Santa Rosa Jauregui 76230, Qro, Mexico

来源：

JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE | 2018年 / 55卷 / 05期

关键词：

Cow's milk; Beta casein A1; Beta casein A2; Polyacrylamide gel; Mass spectrometry; GEL-ELECTROPHORESIS; BETA-CASOMORPHIN-7; QUANTIFICATION; IDENTIFICATION; NOMENCLATURE; PROTEINS; RELEASE;

D O I：

10.1007/s13197-018-3088-z

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Beta-casein (BC) in cow's milk occurs in several genetic variants, where BC A1 (BCA1) and BC A2 (BCA2) are the most frequent. This work deals with a method based on modified polyacrylamide gel electrophoresis using urea PAGE to discriminate BCA1 and BCA2 variants from Holstein Friesian (HF) and genetically selected Jersey A2/A2 (JA2) cow's milk. Two well defined bands were obtained from BC fraction of HF milk, while that of JA2 showed a single band. Proteins from these bands were sequenced by HPLC-quadrupole linear ion trap/mass spectrometry, resulting in BCA1 and BCA2 separation from the BC fraction of HF milk, whereas BCA2 was the only constituent of JA2 fraction. This method represents a feasible and useful tool to on site phenotyping of BC fraction of cow's milk for pharmaceutical and food industries applications.

引用

页码：1942 / 1947

页数：6

共 27 条

[1] PROTEINASES IN NORMAL BOVINE-MILK AND THEIR ACTION ON CASEINS [J].