Noncontact microsurgery of living cell membrane using femtosecond laser pulses

被引：0

作者：

Ilina, I. V. ^{[1
]}

Ovchinnikov, A. V. ^{[1
]}

Sitnikov, D. S. ^{[1
]}

Chefonov, O. V. ^{[1
]}

Agranat, M. B. ^{[1
]}

Mikaelyan, A. S. ^{[2
]}

机构：

[1] RAS, Joint Inst High Temp, Izhorskaya Str 13-2, Moscow 125412, Russia

[2] Russian Acad Sci, Koltzov Inst Dev Biol, Moscow 119334, Russia

来源：

MEDICAL LASER APPLICATIONS AND LASER-TISSUE INTERACTIONS VI | 2013年 / 8803卷

关键词：

femtosecond laser; optoinjection; transfection; cell membrane perforation; living cells; laser microsurgery; TRANSFECTION;

D O I：

10.1117/12.2032545

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Near-infrared femtosecond laser pulses were applied to initiate reversible permeabilization of cell membrane and inject extrinsic substances into the target cells. Successful laser-based injection of a membrane impermeable dye, as well as plasmid DNA was demonstrated.

引用

页数：6

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