Expression and functional role of bTRPC1 channels in native endothelial cells

被引：69
作者：
Antoniotti, S
Lovisolo, D
Pla, AF
Munaron, L
机构：
[1] Univ Turin, Dept Anim & Human Biol, I-10123 Turin, Italy
[2] Udr Torino Univ, INFM, Turin, Italy
来源：
FEBS LETTERS | 2002年 / 510卷 / 03期
关键词：
transient receptor potential-C1; basic fibroblast growth factor; calcium influx; endothelial cells;
D O I：
10.1016/S0014-5793(01)03256-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have analyzed the expression and localization of bovine transient receptor potential-C1 (bTRPC1) in bovine aortic endothelial cells, and its possible involvement in the store-independent calcium influx induced by basic fibroblast growth factor (bFGF). RT-PCR experiments confirmed the existence of two btrpe1 mRNA isoforms; conversely, the btrpc3 gene was not transcribed. Anti-TRPC1 antibody revealed the presence of the protein in the membrane-rich compartment only. Application of anti-TRPC1 during the response to bFGF caused a partial but significant reduction of calcium entry. This is the first evidence of TRP channel involvement in a non-capacitative calcium influx induced by a biologically relevant agonist such as the angiogenic factor bFGF in native endothelial cells. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码：189 / 195
页数：7
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