miR-1306 induces cell apoptosis by targeting BMPR1B gene in the ovine granulosa cells

被引：6

作者：

Abdurahman, Anwar ^{[1
,2
,3
]}

Aierken, Wusimanjiang ^{[4
]}

Zhang, Fei ^{[5
]}

Obulkasim, Rahmantay ^{[6
]}

Aniwashi, Jueken ^{[7
]}

Sulayman, Ablat ^{[8
]}

机构：

[1] Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen Key Lab Marine Bioresources & Ecol, Shenzhen, Peoples R China

[2] Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen, Peoples R China

[3] Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Peoples R China

[4] Xinjiang Med Univ, Affiliate Hosp 5, Urumqi, Peoples R China

[5] Anim Dis Control & Prevent Ctr Xinjiang Uygur Auto, Urumqi, Peoples R China

[6] Ili Kazak Autonomous Prefecture Inst Anim Sci, Ili, Peoples R China

[7] Xinjiang Agr Univ, Coll Anim Sci & Technol, Urumqi, Peoples R China

[8] Xinjiang Acad Anim Sci, Inst Anim Husb, Urumqi, Peoples R China

来源：

FRONTIERS IN GENETICS | 2022年 / 13卷

关键词：

BMPR1B; granulosa cell; miR-1306; apoptosis; sheep; OVULATION RATE; SIGNALING PATHWAY; MIRNAS; PROLIFERATION; EXPRESSION; GROWTH; DIFFERENTIATION; IDENTIFICATION; INVOLVEMENT; MICRORNAS;

D O I：

10.3389/fgene.2022.989912

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Bone morphogenetic protein receptor type-1B (BMPR1B) is one of the major gene for sheep prolificacy. However, few studies investigated its regulatory region. In this study, we reported that miR-1306 is a direct inhibitor of BMPR1B gene in the ovine granulosa cells (ovine GCs). We detected a miRNA response element of miR-1306 in the 3' untranslated region of the ovine BMPR1B gene. Luciferase assay showed that the ovine BMPR1B gene is a direct target of miR-1306. qPCR and western blotting revealed that miR-1306 reduces the expression of BMPR1B mRNA and protein in the ovine granulosa cells. Furthermore, miR-1306 promoted cell apoptosis by suppressing BMPR1B expression in the ovine granulosa cells. Overall, our results suggest that miR-1306 is an epigenetic regulator of BMPR1B, and may serve as a potential target to improve the fecundity of sheep.

引用

页数：9

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