Development of a ratiometric fluorescent pH probe for cell imaging based on a coumarin-quinoline platform

被引：97

作者：

Zhu, Sasa ^{[1
]}

Lin, Weiying ^{[1
]}

Yuan, Lin ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

DYES AND PIGMENTS | 2013年 / 99卷 / 02期

关键词：

Fluorescent probes; Ratiometric; pH; Coumarin; Cellular imaging; Quinoline; INTRACELLULAR PH; ENERGY-TRANSFER; ANION SENSOR; HEPG2;

D O I：

10.1016/j.dyepig.2013.05.010

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

It is highly desirable to develop ratiometric pH probes with a well-resolved emission spectrum for biological imaging applications. In this work the design, synthesis, photophysical properties and cellular imaging studies of a new linked coumarin quinoline probe is described. The major features of the novel probe include two well-resolved (>100 nm) emission peaks, strong fluorescence under acidic conditions, a pKa of 4.69, low cytotoxicity and good cell membrane permeability; features which render the probe useful for monitoring pH variations from neutral to acidic conditions in living cells. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：465 / 471

页数：7

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