Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly

被引：380

作者：

Horton, Edward R. ^{[1
]}

Byron, Adam ^{[1
,2
]}

Askari, Janet A. ^{[1
]}

Ng, Daniel H. J. ^{[1
]}

Millon-Fremillon, Angelique ^{[1
]}

Robertson, Joseph ^{[1
]}

Koper, Ewa J. ^{[1
]}

Paul, Nikki R. ^{[1
]}

Warwood, Stacey ^{[3
]}

Knight, David ^{[3
]}

Humphries, Jonathan D. ^{[1
]}

Humphries, Martin J. ^{[1
]}

机构：

[1] Univ Manchester, Fac Life Sci, Wellcome Trust Ctr Cell Matrix Res, Manchester M13 9PT, Lancs, England

[2] Univ Edinburgh, Inst Genet & Mol Med, Edinburgh Canc Res UK Ctr, Edinburgh EH4 2XR, Midlothian, Scotland

[3] Univ Manchester, Fac Life Sci, Biol Mass Spectrometry Core Facil, Manchester M13 9PT, Lancs, England

来源：

NATURE CELL BIOLOGY | 2015年 / 17卷 / 12期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

TRANSLATION INITIATION-FACTORS; ALPHA-ACTININ; MYOSIN-II; PROTEOMIC ANALYSIS; MESSENGER-RNA; NASCENT ADHESIONS; BINDING PROTEINS; MATRIX ADHESIONS; FOCAL ADHESIONS; CELL-MIGRATION;

D O I：

10.1038/ncb3257

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Integrin receptor activation initiates the formation of integrin adhesion complexes (IACs) at the cell membrane that transduce adhesion-dependent signals to control a multitude of cellular functions. Proteomic analyses of isolated IACs have revealed an unanticipated molecular complexity; however, a global view of the consensus composition and dynamics of IACs is lacking. Here, we have integrated several IAC proteomes and generated a 2,412-protein integrin adhesome. Analysis of this data set reveals the functional diversity of proteins in IACs and establishes a consensus adhesome of 60 proteins. The consensus adhesome is likely to represent a core cell adhesion machinery, centred around four axes comprising ILK PINCH kindlin, FAK paxillin, talin vinculin and alpha-actinin zyxin VASP, and includes underappreciated IAC components such as Rsu-1 and caldesmon. Proteomic quantification of IAC assembly and disassembly detailed the compositional dynamics of the core cell adhesion machinery. The definition of this consensus view of integrin adhesome components provides a resource for the research community.

引用

页码：1577 / 1587

页数：11

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