Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly

被引：392

作者：

Horton, Edward R. ^{[1
]}

Byron, Adam ^{[1
,2
]}

Askari, Janet A. ^{[1
]}

Ng, Daniel H. J. ^{[1
]}

Millon-Fremillon, Angelique ^{[1
]}

Robertson, Joseph ^{[1
]}

Koper, Ewa J. ^{[1
]}

Paul, Nikki R. ^{[1
]}

Warwood, Stacey ^{[3
]}

Knight, David ^{[3
]}

Humphries, Jonathan D. ^{[1
]}

Humphries, Martin J. ^{[1
]}

机构：

[1] Univ Manchester, Fac Life Sci, Wellcome Trust Ctr Cell Matrix Res, Manchester M13 9PT, Lancs, England

[2] Univ Edinburgh, Inst Genet & Mol Med, Edinburgh Canc Res UK Ctr, Edinburgh EH4 2XR, Midlothian, Scotland

[3] Univ Manchester, Fac Life Sci, Biol Mass Spectrometry Core Facil, Manchester M13 9PT, Lancs, England

来源：

NATURE CELL BIOLOGY | 2015年 / 17卷 / 12期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

TRANSLATION INITIATION-FACTORS; ALPHA-ACTININ; MYOSIN-II; PROTEOMIC ANALYSIS; MESSENGER-RNA; NASCENT ADHESIONS; BINDING PROTEINS; MATRIX ADHESIONS; FOCAL ADHESIONS; CELL-MIGRATION;

D O I：

10.1038/ncb3257

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Integrin receptor activation initiates the formation of integrin adhesion complexes (IACs) at the cell membrane that transduce adhesion-dependent signals to control a multitude of cellular functions. Proteomic analyses of isolated IACs have revealed an unanticipated molecular complexity; however, a global view of the consensus composition and dynamics of IACs is lacking. Here, we have integrated several IAC proteomes and generated a 2,412-protein integrin adhesome. Analysis of this data set reveals the functional diversity of proteins in IACs and establishes a consensus adhesome of 60 proteins. The consensus adhesome is likely to represent a core cell adhesion machinery, centred around four axes comprising ILK PINCH kindlin, FAK paxillin, talin vinculin and alpha-actinin zyxin VASP, and includes underappreciated IAC components such as Rsu-1 and caldesmon. Proteomic quantification of IAC assembly and disassembly detailed the compositional dynamics of the core cell adhesion machinery. The definition of this consensus view of integrin adhesome components provides a resource for the research community.

引用

页码：1577 / 1587

页数：11

共 56 条

[1] Novel invadopodia components revealed by differential proteomic analysis [J].

Attanasio, Francesca ;

Caldieri, Giusi ;

Giacchetti, Giada ;

van Horssen, Remco ;

Wieringa, Be ;

Buccione, Roberto .

EUROPEAN JOURNAL OF CELL BIOLOGY, 2011, 90 (2-3) :115-127

[2] Integrin-Associated Complexes Form Hierarchically with Variable Stoichiometry in Nascent Adhesions [J].

Bachir, Alexia I. ;

Zareno, Jessica ;

Moissoglu, Konstadinos ;

Plow, Edward F. ;

Gratton, Enrico ;

Horwitz, Alan R. .

CURRENT BIOLOGY, 2014, 24 (16) :1845-1853

[3] Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration [J].

Bai, Siau Wei ;

Herrera-Abreu, Maria Teresa ;

Rohn, Jennifer L. ;

Racine, Victor ;

Tajadura, Virginia ;

Suryavanshi, Narendra ;

Bechtel, Stephanie ;

Wiemann, Stefan ;

Baum, Buzz ;

Ridley, Anne J. .

BMC BIOLOGY, 2011, 9

[4]

BEGGS AH, 1992, J BIOL CHEM, V267, P9281

[5] The Integrin Co-activator Kindlin-3 Is Expressed and Functional in a Non-hematopoietic Cell, the Endothelial Cell [J].

Bialkowska, Katarzyna ;

Ma, Yan-Qing ;

Bledzka, Kamila ;

Sossey-Alaoui, Khalid ;

Izem, Lahoucine ;

Zhang, Xiaoxia ;

Malinin, Nikolay ;

Qin, Jun ;

Byzova, Tatiana ;

Plow, Edward F. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (24) :18640-18649

[6] Cell adhesion in Drosophila: versatility of cadherin and integrin complexes during development [J].

Bulgakova, Natalia A. ;

Klapholz, Benjamin ;

Brown, Nicholas H. .

CURRENT OPINION IN CELL BIOLOGY, 2012, 24 (05) :702-712

[7] Single β-Actin mRNA Detection in Neurons Reveals a Mechanism for Regulating Its Translatability [J].

Buxbaum, Adina R. ;

Wu, Bin ;

Singer, Robert H. .

SCIENCE, 2014, 343 (6169) :419-422

[8] A proteomic approach reveals integrin activation state-dependent control of microtubule cortical targeting [J].

Byron, Adam ;

Askari, Janet A. ;

Humphries, Jonathan D. ;

Jacquemet, Guillaume ;

Koper, Ewa J. ;

Warwood, Stacey ;

Choi, Colin K. ;

Stroud, Matthew J. ;

Chen, Christopher S. ;

Knight, David ;

Humphries, Martin J. .

NATURE COMMUNICATIONS, 2015, 6

[9] Proteomic analysis of α4β1 integrin adhesion complexes reveals α-subunit-dependent protein recruitment [J].

Byron, Adam ;

Humphries, Jonathan D. ;

Craig, Sue E. ;

Knight, David ;

Humphries, Martin J. .

PROTEOMICS, 2012, 12 (13) :2107-2114

[10] Proteomic Analysis of Integrin Adhesion Complexes [J].

Byron, Adam ;

Humphries, Jonathan D. ;

Bass, Mark D. ;

Knight, David ;

Humphries, Martin J. .

SCIENCE SIGNALING, 2011, 4 (167)

← 1 2 3 4 5 6 →