Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling

被引：39

作者：

Meyer, N. Helge ^{[1
]}

Zangger, Klaus ^{[1
]}

机构：

[1] Graz Univ, Inst Chem, A-8010 Graz, Austria

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 12期

基金：

奥地利科学基金会;

关键词：

SPECTROSCOPY; SEQUENCE;

D O I：

10.1039/c3cc48135b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Limited spectral resolution in the proton dimension of NMR spectra is a severe problem in intrinsically disordered proteins. Here we show that homonuclear broadband proton decoupling of the direct and indirect dimensions of multi-dimensional NMR spectra significantly enhances their resolution.

引用

收藏

页码：1488 / 1490

页数：3

相关论文

共 29 条

[1] Simple Proton Spectra from Complex Spin Systems: Pure Shift NMR Spectroscopy Using BIRD [J].

Aguilar, Juan A. ;

Nilsson, Mathias ;

Morris, Gareth A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (41) :9716-9717

[2] Pure Shift 1H NMR: A Resolution of the Resolution Problem? [J].

Aguilar, Juan A. ;

Faulkner, Stephen ;

Nilsson, Mathias ;

Morris, Gareth A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (23) :3901-3903

[3] HOMONUCLEAR BROAD-BAND DECOUPLING AND 2-DIMENSIONAL J-RESOLVED NMR-SPECTROSCOPY [J].

AUE, WP ;

KARHAN, J ;

ERNST, RR .

JOURNAL OF CHEMICAL PHYSICS, 1976, 64 (10) :4226-4227

[4] INVESTIGATION OF COMPLEX NETWORKS OF SPIN-SPIN COUPLING BY TWO-DIMENSIONAL NMR [J].

BAX, A ;

FREEMAN, R .

JOURNAL OF MAGNETIC RESONANCE, 1981, 44 (03) :542-561

[5] 13C-detected protonless NMR spectroscopy of proteins in solution [J].

Bermel, W ;

Bertini, I ;

Felli, IC ;

Piccioli, M ;

Pierattelli, R .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2006, 48 (01) :25-45

[6] SERF Protein Is a Direct Modifier of Amyloid Fiber Assembly [J].

Falsone, S. Fabio ;

Meyer, N. Helge ;

Schrank, Evelyne ;

Leitinger, Gerd ;

Pham, Chi L. L. ;

Fodero-Tavoletti, Michelle T. ;

Holmberg, Mats ;

Dulle, Martin ;

Scicluna, Benjamin ;

Gesslbauer, Bernd ;

Rueckert, Hanna-Marie ;

Wagner, Gabriel E. ;

Merle, David A. ;

Nollen, Ellen A. ;

Kungl, Andreas J. ;

Hill, Andrew F. ;

Cappai, Roberto ;

Zangger, Klaus .

CELL REPORTS, 2012, 2 (02) :358-371

[7] Recent progress in NMR spectroscopy: Toward the study of intrinsically disordered proteins of increasing size and complexity [J].

Felli, Isabella C. ;

Pierattelli, Roberta .

IUBMB LIFE, 2012, 64 (06) :473-481

[8] The Concept of Spatially-Encoded Single-Scan NMR [J].

Freeman, Ray .

CONCEPTS IN MAGNETIC RESONANCE PART A, 2011, 38A (01) :1-6

[9] BILINEAR ROTATION DECOUPLING OF HOMONUCLEAR SCALAR INTERACTIONS [J].

GARBOW, JR ;

WEITEKAMP, DP ;

PINES, A .

CHEMICAL PHYSICS LETTERS, 1982, 93 (05) :504-509

[10] A general method for diagonal peak suppression in homonuclear correlated NMR spectra by spatially and frequency selective pulses [J].

Glanzer, Simon ;

Schrank, Evelyne ;

Zangger, Klaus .

JOURNAL OF MAGNETIC RESONANCE, 2013, 232 :1-6