Exon Skipping Is Correlated with Exon Circularization

被引：343

作者：

Kelly, Steven ^{[1
,4
]}

Greenman, Chris ^{[2
]}

Cook, Peter R. ^{[3
]}

Papantonis, Argyris ^{[3
,4
]}

机构：

[1] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England

[2] Genome Anal Ctr, Norwich NR4 7UH, Norfolk, England

[3] Univ Oxford, Sir William Dunn Pathol, Oxford OX1 3RE, England

[4] Univ Cologne, Ctr Mol Med, D-50931 Cologne, Germany

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2015年 / 427卷 / 15期

基金：

英国生物技术与生命科学研究理事会;

关键词：

circRNA; cotranscriptional; alternative splicing; turnover; RNAS;

D O I：

10.1016/j.jmb.2015.02.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular RNAs are found in a wide range of organisms and it has been proposed that they perform disparate functions. However, how RNA circularization is connected to alternative splicing remains largely unexplored. Here, we stimulated primary human endothelial cells with tumor necrosis factor a or tumor growth factor 13, purified RNA, generated >2.4 billion RNA-seq reads, and used a custom pipeline to characterize circular RNAs derived from coding exons. We find that circularization of exons is widespread and correlates with exon skipping, a feature that adds considerably to the regulatory complexity of the human transcriptome. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：2414 / 2417

页数：4

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