Glycyrrhizic Acid Ameliorates Mitochondrial Function and Biogenesis Against Aluminum Toxicity in PC12 Cells

Glycyrrhizic acid (GA) is the most effective ingredient in the root of licorice, with important pharmacological effects. We investigate the effects of GA on mitochondrial function and biogenesis in the aluminum toxicity in PC12 cell line. After pretreatment of PC12 cells with different concentrations of GA (5-100M), and specific concentration of aluminum maltolate (Almal,1000M) for 48h, cell viability, reactive oxygen species (ROS), mitochondrial membrane potential (MMP), mitochondria mass, cytochrome c oxidase enzyme activity, and the ATP level of the cells were measured. The expression mRNA level of PGC-1, NRF1, NRF2, and TFAM was confirmed by the real-time PCR quantitative method. The results showed that low concentrations of GA protected Almal-induced cell death in 48h. It was also observed that GA reduced the ROS production and increased the ATP level. The activity of cytochrome c oxidase enzyme and also decrease of MMP were improved. In addition, GA significantly increased the expression of mitochondrial genes and mass against aluminum toxicity. GA can exert its protective effect against the toxicity of Almal through maintaining mitochondrial function and subsequently increasing energy metabolism and mitochondrial biogenesis. GA as a natural product can be considered as a supplement in neurodegenerative disease.