Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome

被引:21
作者
Taube, Amelie Botling [1 ,2 ]
Hardenborg, Emilia [2 ]
Wetterhall, Magnus [2 ]
Artemenko, Konstantin [2 ]
Hanrieder, Jorg [2 ]
Andersson, Marit [2 ]
Alm, Albert [1 ]
Bergquist, Jonas [2 ]
机构
[1] Uppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
[2] Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, Sweden
基金
瑞典研究理事会;
关键词
pseudoexfoliations (PEX); isobaric tagging; protein quantification; proteomics; aqueous humour; osteopontin; angiotensinogen; RENIN-ANGIOTENSIN SYSTEM; RESONANCE MASS-SPECTROMETRY; MALDI-TOF/TOF MS; 5-YEAR FOLLOW-UP; INTRAOCULAR-PRESSURE; LENS CAPSULE; CAPILLARY-ELECTROPHORESIS; EXFOLIATION SYNDROME; CEREBROSPINAL-FLUID; PROTEOMIC ANALYSIS;
D O I
10.1255/ejms.1208
中图分类号
O64 [物理化学(理论化学)、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号
070203 ; 070304 ; 081704 ; 1406 ;
摘要
The aim of this study was to investigate the protein content in aqueous humor in eyes with and without pseudoexfoliations (PEX) and to evaluate the quantitative proteomics method, isobaric tagging for relative and absolute protein quantification (iTRAQ), in combination with two separation methods followed by matrix-assisted Laser desorption/ionization (MALDI) mass spectrometry and tandem mass spectrometry (MS/MS). During cataract surgery, samples of aqueous humor were collected from 20 eyes with PEX and from 18 control eyes. The relative concentrations of proteins in the pooled samples of ten PEX eyes and eight controls were evaluated after trypsin digestion and labeling of the peptides with (iTRAQ) reagent. Two separation methods, Liquid chromatography (LC) and capillary electrophoresis (CE) were used, followed by MALDI mass spectrometry and MS/MS. Furthermore, 1D gel electrophoresis was performed on the remaining ten pooled PEX samples and ten control samples. The gel material was separated by nano-liquid chromatography (nano-LC) followed by Linear-ion-trap quadrupole Fourier transformation ion cyclotron resonance (FT-ICR). Fifty four proteins were identified in the LC runs and 24 with CE. The relative concentrations of beta-crystallines B2 and S were raised and those of angiotensinogen and osteopontin lowered in the PEX sample compared to the control. The trends regarding beta-crystallines B2, angiotensinogen and osteopontin were confirmed by the 1D gel electrophoresis.
引用
收藏
页码:531 / 541
页数:11
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