Proteins in aqueous humor from cataract patients with and without pseudoexfoliation syndrome
被引:21
作者:
Taube, Amelie Botling
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Uppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, SwedenUppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Taube, Amelie Botling
[1
,2
]
Hardenborg, Emilia
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Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, SwedenUppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Hardenborg, Emilia
[2
]
Wetterhall, Magnus
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Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, SwedenUppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Wetterhall, Magnus
[2
]
Artemenko, Konstantin
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Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, SwedenUppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Artemenko, Konstantin
[2
]
Hanrieder, Jorg
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Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, SwedenUppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Hanrieder, Jorg
[2
]
Andersson, Marit
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Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, SwedenUppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Andersson, Marit
[2
]
Alm, Albert
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Uppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, SwedenUppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Alm, Albert
[1
]
Bergquist, Jonas
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Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, SwedenUppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
Bergquist, Jonas
[2
]
机构:
[1] Uppsala Univ, Uppsala Acad Hosp, Dept Neurosci, SE-75185 Uppsala, Sweden
[2] Uppsala Univ, Biomed Ctr, Dept Chem, SE-75124 Uppsala, Sweden
The aim of this study was to investigate the protein content in aqueous humor in eyes with and without pseudoexfoliations (PEX) and to evaluate the quantitative proteomics method, isobaric tagging for relative and absolute protein quantification (iTRAQ), in combination with two separation methods followed by matrix-assisted Laser desorption/ionization (MALDI) mass spectrometry and tandem mass spectrometry (MS/MS). During cataract surgery, samples of aqueous humor were collected from 20 eyes with PEX and from 18 control eyes. The relative concentrations of proteins in the pooled samples of ten PEX eyes and eight controls were evaluated after trypsin digestion and labeling of the peptides with (iTRAQ) reagent. Two separation methods, Liquid chromatography (LC) and capillary electrophoresis (CE) were used, followed by MALDI mass spectrometry and MS/MS. Furthermore, 1D gel electrophoresis was performed on the remaining ten pooled PEX samples and ten control samples. The gel material was separated by nano-liquid chromatography (nano-LC) followed by Linear-ion-trap quadrupole Fourier transformation ion cyclotron resonance (FT-ICR). Fifty four proteins were identified in the LC runs and 24 with CE. The relative concentrations of beta-crystallines B2 and S were raised and those of angiotensinogen and osteopontin lowered in the PEX sample compared to the control. The trends regarding beta-crystallines B2, angiotensinogen and osteopontin were confirmed by the 1D gel electrophoresis.
机构:
Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Sharma, Shiwani
Chataway, Tim
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Flinders Univ S Australia, Dept Human Physiol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Chataway, Tim
Burdon, Kathryn P.
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Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Burdon, Kathryn P.
Jonavicius, Lisa
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Flinders Med Ctr, Dept Anat Pathol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Jonavicius, Lisa
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Klebe, Sonja
Hewitt, Alex W.
论文数: 0引用数: 0
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机构:
Univ Melbourne, Royal Victoria Ear & Eye Hosp, Ctr Eye Res Australia, Melbourne, Vic 3002, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Hewitt, Alex W.
Mills, Richard A.
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机构:
Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Mills, Richard A.
Craig, Jamie E.
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Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
机构:
Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Sharma, Shiwani
Chataway, Tim
论文数: 0引用数: 0
h-index: 0
机构:
Flinders Univ S Australia, Dept Human Physiol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Chataway, Tim
Burdon, Kathryn P.
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h-index: 0
机构:
Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Burdon, Kathryn P.
Jonavicius, Lisa
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机构:
Flinders Med Ctr, Dept Anat Pathol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Jonavicius, Lisa
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Klebe, Sonja
Hewitt, Alex W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Melbourne, Royal Victoria Ear & Eye Hosp, Ctr Eye Res Australia, Melbourne, Vic 3002, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Hewitt, Alex W.
Mills, Richard A.
论文数: 0引用数: 0
h-index: 0
机构:
Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia
Mills, Richard A.
Craig, Jamie E.
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机构:
Flinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, AustraliaFlinders Univ S Australia, Dept Ophthalmol, Bedford Pk, SA 5042, Australia