High-throughput analysis of nucleic acid modification reactions using ion-pair reverse-phase high-performance liquid chromatography

被引：5

作者：

Dickman, MJ ^{[1
]}

Matin, MM ^{[1
]}

Hornby, DP ^{[1
]}

机构：

[1] Univ Sheffield, Krebs Inst, Transgenom Res Lab, Sheffield S10 2TN, S Yorkshire, England

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 301卷 / 02期

关键词：

D O I：

10.1006/abio.2001.5482

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ion-pair reverse-phase high-performance liquid chromatography is presented as a versatile platform for the rapid analysis of nucleic acid modification reactions in a high-throughput manner. This system allows both sensitive and nonradioactive assays to be developed for a variety of nucleic acid modification reactions. Examples presented here include assays for telomerase, uracil DNA glycosylase, polynucleotide kinase, T4 DNA ligase, C5-DNA methyltransferases, and the mismatch endonuclease CEL I. However, this approach is not confined to these reactions. Indeed the ability to perform a variety of nonradioactive assays with throughput times of 10 min per sample in conjunction with automated data analysis software represents a significant improvement in analytical and preparative nucleic acid enzymology. (C) 2002 Elsevier Science (USA).

引用

页码：290 / 297

页数：8

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