Possible implication of Golgi-nucleating function for the centrosome

被引:21
|
作者
Takatsuki, A
Nakamura, M
Kono, Y
机构
[1] RIKEN, Inst Phys & Chem Res, Anim & Cellular Syst Lab, Wako, Saitama 3510198, Japan
[2] Ibaraki Univ, Sch Agr, Ami, Ibaraki 3000332, Japan
关键词
brefeldin A; Golgi apparatus; centrosome; cell cycle; nucleation; nocodazole; microtubule; TGN38; golgin-97; PDMP;
D O I
10.1006/bbrc.2002.6433
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Golgi apparatus breaks down at mitosis, resulting in the dispersal of Golgi-resident proteins. In NRK cells, however, subsets of both TGN38 and golgin-97, but not ManII and GM130, remained associated with the centrosome throughout the cell cycle. This centrosome association of TGN38 and golgin-97 was not disrupted by treatment with brefeldin A, additional inducers of retrograde trafficking and inhibitors of either kinases or protein phosphatases. Anchoring of the Golgi apparatus within the juxtanuclear region depends on microtubules; the association of TGN38 and golgin-97 subsets with the centrosome, however, was insensitive to nocodazole treatment. Drugs such as PDMP, which block Golgi dispersal both by nocodazole, despite microtubule depolymerization, and by inducers of retrograde trafficking, strengthened the microtubule-nucleating activity of the centrosome. These observations cumulatively suggest the centrosome is implicated in nucleation of the Golgi apparatus through interactions with Golgi-resident proteins, such as TGN38 and golgin-97. (C) 2002 Elsevier Science (USA).
引用
收藏
页码:494 / 500
页数:7
相关论文
共 50 条
  • [31] GRASPs in Golgi Structure and Function
    Zhang, Xiaoyan
    Wang, Yanzhuang
    FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2016, 3
  • [32] Human centrosome organization and function in interphase and mitosis
    Nunes, Vanessa
    Ferreira, Jorge G.
    SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2021, 117 : 42 - 51
  • [33] ER-to-Golgi Trafficking and Its Implication in Neurological Diseases
    Wang, Bo
    Stanford, Katherine R.
    Kundu, Mondira
    CELLS, 2020, 9 (02)
  • [34] The mechanism of granulocyte nuclear shape determination: possible involvement of the centrosome
    Olins, AL
    Olins, DE
    EUROPEAN JOURNAL OF CELL BIOLOGY, 2005, 84 (2-3) : 181 - 188
  • [35] The centrosome keeps nucleating microtubules but looses the ability to anchor them after the inhibition of dynein-dynactin complex
    Zhapparova, O. N.
    Burakov, A. V.
    Nadezhdina, E. S.
    BIOCHEMISTRY-MOSCOW, 2007, 72 (11) : 1233 - 1240
  • [36] The centrosome keeps nucleating microtubules but looses the ability to anchor them after the inhibition of dynein-dynactin complex
    O. N. Zhapparova
    A. V. Burakov
    E. S. Nadezhdina
    Biochemistry (Moscow), 2007, 72 : 1233 - 1240
  • [37] Melting and solidification of thermoplastic polyurethanes as a function of nucleating agents
    Stribeck, Almut
    Eling, Berend
    Poeselt, Elmar
    Malfois, Marc
    Schander, Edgar
    NANO SELECT, 2022, 3 (03): : 673 - 687
  • [38] Centrosome function is critical during terminal erythroid differentiation
    Tatrai, Peter
    Gergely, Fanni
    EMBO JOURNAL, 2022, 41 (14)
  • [39] A possible role for centrosome overduplication in radiation-induced cell death
    Sato, N
    Mizumoto, K
    Nakamura, M
    Ueno, H
    Minamishima, YA
    Farber, JL
    Tanaka, M
    ONCOGENE, 2000, 19 (46) : 5281 - 5290
  • [40] Possible implication of the Golgi apparatus casein kinase in the phosphorylation of vesicle docking protein p115 Ser-940: A study with peptide substrates
    Brunati, AM
    Marin, O
    Folda, A
    Meggio, F
    Pinna, LA
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 284 (03) : 817 - 822