Imperatorin Attenuates LPS-Induced Inflammation by Suppressing NF-κB and MAPKs Activation in RAW 264.7 Macrophages

被引:69
作者
Guo, Weixiao [1 ]
Sun, Jingjing [1 ]
Jiang, Lanxiang [2 ]
Duan, Lingxin [3 ]
Huo, Meixia [1 ]
Chen, Na [1 ]
Zhong, Weiting [1 ]
Wassy, Lanan [1 ]
Yang, Zhenguo [1 ]
Feng, Haihua [1 ]
机构
[1] Jilin Univ, Dept Vet Pharmacol, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China
[2] Jilin Univ, Hosp 2, Dept Dermatol, Changchun 130041, Peoples R China
[3] Hebei Normal Univ Sci & Technol, Qinhuangdao 066600, Peoples R China
基金
中国博士后科学基金;
关键词
imperatorin; cytokines; NF-kappa B; MAPK; NITRIC-OXIDE SYNTHASE; TOLL-LIKE RECEPTOR; CYTOKINE PRODUCTION; KINASE PATHWAYS; LIPOPOLYSACCHARIDE; P38; MEDIATORS; ISCHEMIA; CELL;
D O I
10.1007/s10753-012-9495-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Imperatorin is a type of coumarin compound with antibacterial and antiviral activities. In the present study, we examined the anti-inflammatory effects of imperatorin in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages by investigating its impact on the production and expression of cytokines and the major signal-transduction pathways. We found that imperatorin downregulated LPS-induced levels of TNF-alpha, IL-1 beta, and IL-6 in RAW 264.7 macrophages in a concentration-dependent manner, and it significantly inhibited expression of TNF-alpha and IL-6 (P < 0.05 or P < 0.01). The phosphorylation of mitogen-activated protein kinases and nuclear factor-kappaB (NF-kappa B) p65 protein were analyzed by western blotting. In RAW 264.7 macrophages treated with 1 mg/L of LPS, imperatorin significantly inhibited p38 and Jun N-terminal kinase phosphorylation protein expression. However, there was no significant change in p-ERK. Furthermore, imperatorin also inhibited NF-kappa B translocation into the nucleus through blockage of I kappa B alpha phosphorylation and degradation.
引用
收藏
页码:1764 / 1772
页数:9
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