N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation

被引：5

作者：

Noh, Y. -H. ^{[1
]}

Cho, H. -S. ^{[2
]}

Kim, D. -H. ^{[1
]}

Kim, O. H. ^{[1
]}

Park, J. ^{[1
]}

Lee, S. -A. ^{[1
]}

Yang, H. -S. ^{[2
]}

Sohn, D. -S. ^{[3
]}

Kim, W. ^{[4
]}

Kim, D. ^{[1
]}

Chung, Y. H. ^{[1
]}

Kim, K. Y. ^{[1
]}

Kim, S. S. ^{[1
]}

Lee, W. B. ^{[1
]}

机构：

[1] Chung Ang Univ, Coll Med, Dept Cell Biol & Anat, Seoul 156756, South Korea

[2] Chung Ang Univ, Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Seoul 156756, South Korea

[3] Chung Ang Univ, Coll Med, Dept Thorac Surg, Seoul 156756, South Korea

[4] Chung Ang Univ, Coll Med, Dept Microbiol, Seoul 156756, South Korea

来源：

MOLECULAR BIOLOGY | 2012年 / 46卷 / 05期

关键词：

Akt; N-cadherin; N-acetylcysteine; P19 embryonic stem cell; differentiation; CARCINOMA-CELLS; EXPRESSION; PATHWAY; PROTEIN; BRAIN;

D O I：

10.1134/S0026893312040085

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.

引用

页码：664 / 669

页数：6

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