Genomic in situ hybridization in interspecific hybrids of scallops (Bivalvia, Pectinidae) and localization of the satellite DNA Cf303, and the vertebrate telomeric sequences (TTAGGG)n on chromosomes of scallop Chlamys farreri (Jones & Preston, 1904)

被引：1
作者：
Hu, Liping [1 ,2 ]
Jiang, Liming [1 ]
Bi, Ke [3 ]
Liao, Huan [1 ]
Yang, Zujing [1 ]
Huang, Xiaoting [1 ]
Bao, Zhenmin [1 ,4 ]
机构：
[1] Ocean Univ China, Coll Marine Life Sci, Minist Educ, Key Lab Marine Genet & Breeding, 5 Yushan Rd, Qingdao 266003, Peoples R China
[2] Yantai Fisheries Res Inst, Yantai 264003, Peoples R China
[3] Univ Calif Berkeley, Museum Vertebrate Zool, Berkeley, CA 94720 USA
[4] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
来源：
COMPARATIVE CYTOGENETICS | 2018年 / 12卷 / 01期
基金：
中国国家自然科学基金;
关键词：
GISH banding; FISH; Cf303; telomere; scallop; CYTOGENETIC CHARACTERIZATION; REPETITIVE DNA; DONAX-TRUNCULUS; PACIFIC OYSTER; ARGOPECTEN-IRRADIANS; AEGILOPS-SPELTOIDES; STAINING TECHNIQUES; PARENTAL GENOMES; RIBOSOMAL DNA; EVOLUTION;
D O I：
10.3897/CompCytogen.v12i1.14995
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Mitotic chromosome preparations of the interspecific hybrids Chlamys farreri (Jones & Preston, 1904) x Patinopecten yessoensis (Jay, 1857), C. farreri x Argopecten irradinas (Lamarck, 1819) and C. farreri x Mimachlamys nobilis (Reeve, 1852) were used to compare two different scallop genomes in a single slide. Although genomic in situ hybridization (GISH) using genomic DNA from each scallop species as probe painted mitotic chromosomes of the interspecific hybrids, the painting results were not uniform; instead it showed species-specific distribution patterns of fluorescent signals among the chromosomes. The most prominent GISH-bands were mainly located at centromeric or telomeric regions of scallop chromosomes. In order to illustrate the sequence constitution of the GISH-bands, the satellite Cf303 sequences of C. farreri and the vertebrate telomeric (TTAGGG)(n) sequences were used to map mitotic chromosomes
引用
收藏
页码：83 / 95
页数：13
相关论文
共 65 条
[1]   Heterochromatin discrimination in Aegilops speltoides by simultaneous genomic in situ hybridization [J].
Belyayev, A ;
Raskina, O .
CHROMOSOME RESEARCH, 1998, 6 (07) :559-565
[2]  
Belyayev A, 2001, HEREDITY, V86, P738, DOI 10.1046/j.1365-2540.2001.00891.x
[3]  
Boron A, 2004, FOLIA BIOL-KRAKOW, V52, P33
[4]   MOLECULAR ARRANGEMENT AND EVOLUTION OF HETEROCHROMATIC DNA [J].
BRUTLAG, DL .
ANNUAL REVIEW OF GENETICS, 1980, 14 :121-144
[5]   Physical Mapping by FISH and GISH of rDNA Loci and Discrimination of Genomes A and B in Scilla scilloides Complex Distributed in Korea [J].
Choi, Hae-Woon ;
Kim, Jeong-Soon ;
Lee, Sang-Hoon ;
Bang, Jae-Wook .
JOURNAL OF PLANT BIOLOGY, 2008, 51 (06) :408-412
[6]   Cloning, characterization and chromosomal location of a satellite DNA from the Pacific oyster, Crassostrea gigas [J].
Clabby, C ;
Goswami, U ;
Flavin, F ;
Wilkins, NP ;
Houghton, JA ;
Powell, R .
GENE, 1996, 168 (02) :205-209
[7]   FLUORESCENT IN-SITU HYBRIDIZATION AND C-BANDING ANALYSES OF HIGHLY REPETITIVE DNA-SEQUENCES IN THE HETEROCHROMATIN OF RYE (SECALE-MONTANUM GUSS) AND WHEAT INCORPORATING SECALE-MONTANUM CHROMOSOME SEGMENTS [J].
CUADRADO, A ;
JOUVE, N .
GENOME, 1995, 38 (04) :795-802
[8]   REPETITIVE DNA AND CHROMOSOME EVOLUTION IN PLANTS [J].
FLAVELL, RB .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1986, 312 (1154) :227-242
[9]   Methylation profile of a satellite DNA constituting the intercalary G plus C-rich heterochromatin of the cut trough shell Spisula subtruncata (Bivalvia, Mactridae) [J].
Garcia-Souto, Daniel ;
Mravinac, Brankica ;
Satovic, Eva ;
Plohl, Miroslav ;
Moran, Paloma ;
Pasantes, Juan J. .
SCIENTIFIC REPORTS, 2017, 7
[10]   Molecular Cytogenetics in Trough Shells (Mactridae, Bivalvia): Divergent GC-Rich Heterochromatin Content [J].
Garcia-Souto, Daniel ;
Perez-Garcia, Concepcion ;
Kendall, Jack ;
Pasantes, Juan J. .
GENES, 2016, 7 (08)
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