Preanalytical and Analytical Considerations for Meastutriing Nitric Oxide Metabolites in Serum or Plasma Using the Griess Method

被引：43
作者：
Ghasemi, Asghar [1 ]
Zahediasl, Saleh [1 ]
机构：
[1] Shahid Beheshti Univ Med Sci, Res Inst Endocrine Sci, Endocrine Res Ctr, Tehran, Iran
来源：
CLINICAL LABORATORY | 2012年 / 58卷 / 7-8期
关键词：
Griess assay; nitric oxide; nitrates; nitrites; plasma; quality control; serum; CHROMATOGRAPHY-MASS-SPECTROMETRY; PERFORMANCE-LIQUID-CHROMATOGRAPHY; INEXPENSIVE AUTOMATED TECHNIQUE; BIOLOGICAL-FLUIDS; NITRITE/NITRATE CONCENTRATION; SPECTROPHOTOMETRIC METHOD; INORGANIC NITRATE; END-PRODUCTS; HUMANS; ASSAY;
D O I：
10.7754/Clin.Lab.2011.110908
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Nitric oxide (NO) plays numerous important roles in many biological systems and, therefore, research in this field, which impacts all aspects of biology and medicine, especially NO measurement, is steadily increasing. Nitric oxide has a short biological half-life, making its direct determination in vivo difficult. Therefore, currently assessment of nitrite and nitrate (NOx) in biological fluids seems to be the most suitable method to assess NO synthesis in vivo. The most frequently used method of NOx analysis is based on the Griess reaction. Although measurement of NOx by the Griess assay is simple and cheap, the accurate determination of plasma/serum concentrations of nitrate/nitrite using the Griess assay is prone to interferences and can be influenced by several factors. The aim of this study was to overview preanalytical and analytical considerations for measuring NOx in serum or plasma using the Griess assay. (Clin. Lab.2012;58:615-624. DOI: 10.7754/Clin.Lab.2011.110908)
引用
收藏
页码：615 / 624
页数：10
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