Ionic liquids as alternative co-solvents for laccase: Study of enzyme activity and stability

被引：75

作者：

Mora T'avares, Ana Paula ^{[1
]}

Rodriguez, Oscar ^{[1
]}

Macedo, Eugenia A. ^{[1
]}

机构：

[1] Univ Porto, Fac Engn, Dept Engn Quim, LSRE, P-4200465 Oporto, Portugal

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2008年 / 101卷 / 01期

关键词：

ionic liquids; laccase; kinetic parameters; organic solvents; stability;

D O I：

10.1002/bit.21866

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The activity and stability of commercial laccase (DeniLite base) in three different water soluble ionic liquids (ILs) (1-ethyl-3-methylimidazolium 2-(2-methoxyethoxy) ethylsulfate, [emim][MIDEGSO(4)], 1-ethyl-3-methylimidazolium ethylsulfate, [emim][EtSO4], and 1-ethyl-3-methylimidazolium methanesulfonate, [ernim][MeSO3]) have been Studied and compared to that in two organic solvents (acetonitrile and dimethyl sulfoxide). Initial enzyme activities were similar among the ILs if the same conditions were used. A high reduction on initial enzyme activity was found with acidic pH (5.0). The effect of pH and solvent concentration on enzyme stability were investigated in more detail for I week. The enzyme maintained a high stability at pH 9.0 for all ILs tested. [emim] [MDEGSO(4)] was the most promising IL for laccase with an activity loss of about 10% after 7 days of incubation. The kinetic studies in the presence of ABTS as substrate allowed to calculate the Michaelis-Menten parameters. Good agreement was found between experimental data and calculated values using the Michaelis-Menten mechanism, with a total average relative deviation of 2.1 %. Biotechnol. Bioeng. 2008;101: 201-207. (C) 2008 Wiley Periodicals, Inc.

引用

页码：201 / 207

页数：7

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