Ionic liquids as alternative co-solvents for laccase: Study of enzyme activity and stability

被引：75

作者：

Mora T'avares, Ana Paula ^{[1
]}

Rodriguez, Oscar ^{[1
]}

Macedo, Eugenia A. ^{[1
]}

机构：

[1] Univ Porto, Fac Engn, Dept Engn Quim, LSRE, P-4200465 Oporto, Portugal

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2008年 / 101卷 / 01期

关键词：

ionic liquids; laccase; kinetic parameters; organic solvents; stability;

D O I：

10.1002/bit.21866

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The activity and stability of commercial laccase (DeniLite base) in three different water soluble ionic liquids (ILs) (1-ethyl-3-methylimidazolium 2-(2-methoxyethoxy) ethylsulfate, [emim][MIDEGSO(4)], 1-ethyl-3-methylimidazolium ethylsulfate, [emim][EtSO4], and 1-ethyl-3-methylimidazolium methanesulfonate, [ernim][MeSO3]) have been Studied and compared to that in two organic solvents (acetonitrile and dimethyl sulfoxide). Initial enzyme activities were similar among the ILs if the same conditions were used. A high reduction on initial enzyme activity was found with acidic pH (5.0). The effect of pH and solvent concentration on enzyme stability were investigated in more detail for I week. The enzyme maintained a high stability at pH 9.0 for all ILs tested. [emim] [MDEGSO(4)] was the most promising IL for laccase with an activity loss of about 10% after 7 days of incubation. The kinetic studies in the presence of ABTS as substrate allowed to calculate the Michaelis-Menten parameters. Good agreement was found between experimental data and calculated values using the Michaelis-Menten mechanism, with a total average relative deviation of 2.1 %. Biotechnol. Bioeng. 2008;101: 201-207. (C) 2008 Wiley Periodicals, Inc.

引用

页码：201 / 207

页数：7

共 38 条

[1] Decolorization of dyes from textile wastewater by Trametes versicolor [J].

Amaral, PFF ;

Fernandes, DLA ;

Tavares, APM ;

Xavier, ABMR ;

Cammarota, MC ;

Coutinho, JAP ;

Coelho, MAZ .

ENVIRONMENTAL TECHNOLOGY, 2004, 25 (11) :1313-1320

[2] Oxidation of 1-hydroxybenzotriazole by laccase and lignin peroxidase [J].

Ander, P ;

Messner, K .

BIOTECHNOLOGY TECHNIQUES, 1998, 12 (03) :191-195

[3] Effect of water activity on the lipase catalyzed esterification of geraniol in ionic liquid [bmim]PF6 [J].

Barahona, D ;

Pfromm, PH ;

Rezac, ME .

BIOTECHNOLOGY AND BIOENGINEERING, 2006, 93 (02) :318-324

[4] Phosphonium ionic liquids for degradation of phenol in a two-phase partitioning bioreactor [J].

Baumann, MD ;

Daugulis, AJ ;

Jessop, PG .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2005, 67 (01) :131-137

[5] Water activity fails to predict critical hydration level for enzyme activity in polar organic solvents: Interconversion of water concentrations and activities [J].

Bell, G ;

Janssen, AEM ;

Halling, PJ .

ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (06) :471-477

[6] Ionic liquids: Innovative fluids for chemical processing [J].

Brennecke, JF ;

Maginn, EJ .

AICHE JOURNAL, 2001, 47 (11) :2384-2389

[7] Bioremediation of food industry effluents: Recent applications of free and immobilised polyphenoloxidases [J].

Chiacchierini, E ;

Restuccia, D ;

Vinci, G .

FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL, 2004, 10 (06) :373-382

[8] Effect of ionic liquids on epoxide hydrolase-catalyzed synthesis of chiral 1,2-diols [J].

Chiappe, Cinzia ;

Leandri, Elsa ;

Hammock, Bruce D. ;

Morisseau, Christophe .

GREEN CHEMISTRY, 2007, 9 (02) :162-168

[9] Understanding structure -: Stability relationships of Candida antartica lipase B in ionic liquids [J].

De Diego, T ;

Lozano, P ;

Gmouh, S ;

Vaultier, M ;

Iborra, JL .

BIOMACROMOLECULES, 2005, 6 (03) :1457-1464

[10] Ionic liquids. Green solvents for the future [J].

Earle, MJ ;

Seddon, KR .

PURE AND APPLIED CHEMISTRY, 2000, 72 (07) :1391-1398

← 1 2 3 4 →