Effects of P27/Bmdacapo, in the CIP/KIP family, on cell proliferation, growth and development in the silkworm (Bombyx mori)

被引：1

作者：

Li, Niannian ^{[1
]}

Meng, Gang ^{[1
]}

Tong, Xiaoling ^{[1
]}

Sun, Fuze ^{[1
]}

Zeng, Jie ^{[1
]}

Bai, Yanmin ^{[1
]}

Liang, Shubo ^{[1
]}

Hu, Hai ^{[1
]}

Liu, Lanlan ^{[1
]}

Han, Minjin ^{[1
]}

Lu, Cheng ^{[1
]}

Dai, Fangyin ^{[1
]}

机构：

[1] Southwest Univ, Minist Agr, Key Lab Sericultural Biol & Genet Breeding, Coll Biotechnol,State Key Lab Silkworm Genome Bio, Chongqing 400715, Peoples R China

来源：

GENE | 2019年 / 700卷

基金：

中国国家自然科学基金;

关键词：

P27/Dacapo; Silkworm; CIP/KIP family; Gu/S; Cell cycle; Development; DEPENDENT KINASE INHIBITOR; IN-VIVO; CYCLIN; P27; EXPRESSION; P27(KIP1); APOPTOSIS; ARREST; DACAPO; LUNG;

D O I：

10.1016/j.gene.2019.02.087

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We investigated changes in expression of the CIP/KIP family-related genes and the cycle-dependent factors Pena, Cdk4 and Cdk2 during the growth and development of mice, Drosophila and silkworms. When the organism was in a period of rapid development, the related genes of the CIP/KIP family had low expression level and the cell cycle-dependent genes were highly expressed. In mammals, the CIP/KIP family includes three genes, p21, p27/Dacapo and p57. However, only one gene, P27/Dacapo, exists in the CIP/KIP family in silkworm' and the orthologous gene in the silkworm is named Bmdacapo. Down-regulation of Bmdacapo in silkworm embryos caused overdevelopment of the embryos and indicated that Bmdacapo can inhibit silkworm growth and development. Up-regulation of Bmdacapo in silkworm cells inhibited cell proliferation, whereas down-regulation of Bmdacapo promoted cell proliferation. In order to explore the mechanism of Bmdacapo regulated silkworm development and cell proliferation, the effect of Bmdacapo on cell cycle changes was examined. The results demonstrate that Bmdacapo was able to induce G1/S phase arrest in the cell cycle. In silkworm cells, Bmdacapo inhibits the expression of Pcna, CDK4 and CDK2, which affects the cell cycle and ultimately inhibits cell proliferation. This regulatory mechanism is particularly different from mammals.

引用

页码：31 / 37

页数：7

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