Fused Deposition Modeling 3D Printing: Test Platforms for Evaluating Post-Fabrication Chemical Modifications and In-Vitro Biological Properties

被引:14
作者
Arany, Petra [1 ]
Roka, Eszter [1 ,2 ]
Mollet, Laurent [3 ]
Coleman, Anthony W. [3 ]
Perret, Florent [2 ]
Kim, Beomjoon [4 ]
Kovacs, Renato [5 ,6 ]
Kazsoki, Adrienn [7 ]
Zelko, Romana [7 ]
Gesztelyi, Rudolf [8 ]
Ujhelyi, Zoltan [1 ]
Feher, Palma [1 ]
Varadi, Judit [1 ]
Fenyvesi, Ferenc [1 ]
Vecsernyes, Miklos [1 ]
Bacskay, Ildiko [1 ]
机构
[1] Univ Debrecen, Fac Pharm, Dept Pharmaceut Technol, Nagyerdei Korut 98, H-4032 Debrecen, Hungary
[2] Univ Lyon 1, ICBMS, UMR 5246, F-69622 Villeurbanne, France
[3] Univ Lyon 1, LMI CNRS UMR 5615, F-69622 Villeurbanne, France
[4] Univ Tokyo, Inst Ind Sci, LIMMS CNRS IIS UMI 2820, Tokyo 1538505, Japan
[5] Univ Debrecen, Dept Med Microbiol, Fac Med, Nagyerdei Korut 98, H-4032 Debrecen, Hungary
[6] Univ Debrecen, Fac Pharm, Nagyerdei Korut 98, H-4032 Debrecen, Hungary
[7] Univ Semmelweis, Univ Pharm Dept Pharm Adm, Fac Pharm, Hogyes Endre Utca 7-9, H-1092 Budapest, Hungary
[8] Univ Debrecen, Dept Pharmacol & Pharmacotherapy, Nagyerdei Korut 98, H-4032 Debrecen, Hungary
来源
PHARMACEUTICS | 2019年 / 11卷 / 06期
关键词
fused deposition modeling; polylactic acid; chemical modification; MTT assay; biofilm formation; POLYLACTIC ACID; MTT ASSAY; BIOCOMPATIBILITY; SCAFFOLDS; SURFACE; CELL; CYTOTOXICITY; PLA; BIODEGRADATION; COMPOSITE;
D O I
10.3390/pharmaceutics11060277
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
3D printing is attracting considerable interest for its capacity to produce prototypes and small production runs rapidly. Fused deposit modeling (FDM) was used to produce polyvalent test plates for investigation of the physical, chemical, and in-vitro biological properties of printed materials. The polyvalent test plates (PVTPs) are poly-lactic acid cylinders, 14 mm in diameter and 3 mm in height. The polymer ester backbone was surface modified by a series of ramified and linear oligoamines to increase its hydrophilicity and introduce a positive charge. The chemical modification was verified by FT-IR spectroscopy, showing the introduction of amide and amine functions, and contact angle measurements confirmed increased hydrophilicity. Morphology studies (SEM, optical microscopy) indicated that the modification of PVTP possessed a planar morphology with small pits. Positron annihilation lifetime spectroscopy demonstrated that the polymeric free volume decreased on modification. An MTT-based prolonged cytotoxicity test using Caco-2 cells showed that the PVTPs are non-toxic at the cellular level. The presence of surface oligoamines on the PVTPs reduced biofilm formation by Candida albicans SC5314 significantly. The results demonstrate that 3D printed objects may be modified at their surface by a simple amidation reaction, resulting in a reduced propensity for biofilm colonization and cellular toxicity.
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页数:23
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