Extraction, Purification and Nanoformulation of Natural Phycocyanin (from Klamath algae) for Dermal and Deeper Soft Tissue Delivery

被引:18
作者
Caddeo, Carla [1 ]
Chessa, Maura [1 ]
Vassallo, Antonio [2 ]
Pons, Ramon [3 ]
Diez-Sales, Octavio [4 ]
Fadda, Anna Maria [1 ]
Manconi, Maria [1 ]
机构
[1] Univ Cagliari, Dept Sci Vita & Ambiente, Sez Sci Farmaco, I-09124 Cagliari, Italy
[2] Univ Basilicata, Dept Sci, I-85100 Potenza, Italy
[3] CSIC, IQAC, Dept Tecnol Quim & Tensioactius, ES-08034 Barcelona, Spain
[4] Univ Valencia, Dept Pharm & Pharmaceut Technol, E-46100 Valencia, Spain
关键词
Phycocyanin; Protein Extraction and Purification; Phospholipid Vesicle; Topical Delivery; Human Skin; VITRO PERCUTANEOUS PERMEATION; APHANIZOMENON-FLOS-AQUAE; IN-VIVO EVALUATION; C-PHYCOCYANIN; PENETRATION ENHANCER; SKIN PENETRATION; ANTIINFLAMMATORY ACTIVITY; SPIRULINA-PLATENSIS; LIPOSOMES; CARRIERS;
D O I
10.1166/jbn.2013.1741
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
This study focuses on the extraction and isolation of a natural anti-inflammatory phycocyanin and its nanofornnulation in innovative and efficient vesicular carriers able to improve its delivery to the skin. C-phycocyanin was successfully isolated from a commercial dry extract (AfaMax (R)) of blue-green Klamath algae. Protein extraction and purity were confirmed by gel electrophoresis (SDS PAGE), MALDI top-down sequencing, Liquid Chromatography/Mass Spectrometry, and UV absorption. Purified C-phycocyanin was then encapsulated in different phospholipid vesicles: liposomes, ethosomes and Penetration Enhancer containing Vesicles (PEVs), the latter containing the penetration enhancer propylene glycol or Transcutol P. The main colloidal characteristics of the systems were assessed, showing spherical vesicles around 100 nm, negatively charged, with different lamellarity depending on the formulation composition. An in depth investigation on vesicle geometrical properties and morphology was carried out by Small and Wide-Angle X-ray Scattering. Further, the ability of rhodamine-labelled vesicles to allow fluorescent phycocyanin penetration and distribution through human skin was evaluated by Confocal Laser Scanning Microscopy, while a complete picture of vesicle-treated skin architecture was gained using Scanning Electron Microscopy. Results indicate that PEVs, especially propylene glycol containing vesicles, are promising carriers for the delivery of the high molecular weight protein phycocyanin to the deep skin layers.
引用
收藏
页码:1929 / 1938
页数:10
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