Determination of 20(S)-protopanaxadiol in rat plasma by LC-MS/MS and its application to the pharmacokinetic study: A comparative study of its solution and two oral formulations

被引：7

作者：

Han, Meihua ^{[1
,2
]}

Chen, Jing ^{[3
]}

Wang, Yuqin ^{[4
]}

Chen, Shilin ^{[1
,2
]}

Wang, Xiangtao ^{[1
,2
]}

机构：

[1] Chinese Acad Med Sci, Beijing 100193, Peoples R China

[2] Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China

[3] Harbin Univ Commerce, Life Sci & Environm Sci Res Ctr, Engn Res Ctr Nat Anticanc Drugs, Minist Educ, Harbin 150076, Peoples R China

[4] SIRC TCM, Shanghai 201203, Peoples R China

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2013年 / 68卷 / 08期

关键词：

20(S)-protopanaxadiol; high-performance liquid chromatography. tandem mass spectrometry method; MASS-SPECTROMETRY; GINSENOSIDE RG3; CELLS; PROLIFERATION; METABOLITES; RH2;

D O I：

10.1134/S106193481308008X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatography - tandem mass spectrometry method was used in a comparative pharmacokinetic study on 20(S)-protopanaxadiol in its solution, pharmacosomes and hydroxy-propyl-beta-cyclodextrin inclusion compound. The calibration linearity range was 2.5-5000 ng/mL and the limit of quantification was 2.5 ng/mL. The intra- and inter-assay coefficients of variation were less than 7% and the recoveries were 67.4, 63.3 and 69.6% at 5, 250 and 4500 ng/mL. In contrast to 20(S)-protopanaxadiol solution, its pharmacosomes and hydroxypropyl-beta-cyclodextrin inclusion compound improved the oral bioavailability to different extent and meanwhile were much safer in clinical use.

引用

页码：730 / 735

页数：6

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