Specific primer design for the polymerase chain reaction

被引：50

作者：

Chuang, Li-Yeh ^{[1
]}

Cheng, Yu-Huei ^{[2
]}

Yang, Cheng-Hong ^{[3
]}

机构：

[1] I Shou Univ, Dept Chem Engn, Inst Biotechnol & Chem Engn, Kaohsiung, Taiwan

[2] Toko Univ, Dept Digital Content Design & Management, Chiayi, Taiwan

[3] Natl Kaohsiung Univ Appl Sci, Dept Elect Engn, Kaohsiung 807, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 10期

关键词：

Algorithm; Computational intelligence; Polymerase chain reaction; Primer design; ENZYMATIC AMPLIFICATION; PCR; TOOL; ALGORITHM; IDENTIFICATION; SEQUENCES; SUCCESS;

D O I：

10.1007/s10529-013-1249-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The design of primers has a major impact on the success of PCR in relation to the specificity and yield of the amplified product. Here, we introduce the applications of PCR as well as the definition and characteristics for PCR primer design. Recent primer design tools based on Primer3, along with several computational intelligence-based primer design methods which have been applied in primer design, are also reviewed. In addition, characteristics of population-based methods used in primer design are discussed in detail.

引用

页码：1541 / 1549

页数：9

共 54 条

[1] Novel Memetic Algorithm implemented With GA (Genetic Algorithm) and MADS (Mesh Adaptive Direct Search) for Optimal Design of Electromagnetic System [J].