Dosimetry of N6-Formyllysine Adducts Following [13C2H2]-Formaldehyde Exposures in Rats

被引：28

作者：

Edrissi, Bahar ^{[1
]}

Taghizadeh, Koli ^{[2
]}

Moeller, Benjamin C. ^{[3
,4
]}

Kracko, Dean ^{[4
]}

Doyle-Eisele, Melanie ^{[4
]}

Swenberg, James A. ^{[3
]}

Dedon, Peter C. ^{[1
,2
]}

机构：

[1] MIT, Dept Biol Engn, Cambridge, MA 02139 USA

[2] MIT, Ctr Environm Hlth Sci, Cambridge, MA 02139 USA

[3] Univ N Carolina, Dept Environm Sci & Engn, Chapel Hill, NC 27514 USA

[4] Lovelace Resp Res Inst, Albuquerque, NM 87108 USA

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 2013年 / 26卷 / 10期

关键词：

FORMYLATION; PROTEINS; LYSINE;

D O I：

10.1021/tx400320u

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

With formaldehyde as the major source of endogenous N-6-formyllysine protein adducts, we quantified endogenous and exogenous N6-formyllysine in the nasal epithelium of rats exposed by inhalation to 0.7, 2, 5.8, and 9.1 ppm [(CH2)-C-15-H-2]-formaldehyde using liquid chromatography-coupled tandem mass spectrometry. Exogenous N-6-formyllysine was detected in the nasal epithelium, with concentration-dependent formation in total as well as fractionated (cytoplasmic, membrane, nuclear) proteins, but was not detected in the lung, liver, or bone marrow. Endogenous adducts dominated at all exposure conditions, with a 6 h 9.1 ppm formaldehyde exposure resulting in one-third of the total load of N-6-formyllysine being derived from exogenous sources. The results parallel previous studies of formaldehyde-induced DNA adducts.

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收藏

页码：1421 / 1423

页数：3

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