Dopamine transporter neuroimaging accurately assesses the maturation of dopamine neurons in a preclinical model of Parkinson's disease

被引：9

作者：

Goggi, Julian L. ^{[1
]}

Qiu, Lifeng ^{[2
]}

Liao, Mei Chih ^{[3
]}

Khanapur, Shivashankar ^{[1
]}

Jiang, Lingfan ^{[1
]}

Boominathan, Ramasamy ^{[1
]}

Hartimath, Siddesh, V ^{[1
]}

Cheng, Peter ^{[1
]}

Yong, Fui Fong ^{[1
]}

Soh, Vanessa ^{[1
]}

Deng, Xiaozhou ^{[1
]}

Lin, Youshan Melissa ^{[3
]}

Haslop, Anna ^{[1
]}

Tan, Peng Wen ^{[1
]}

Zeng, Xiaoxia ^{[2
]}

Lee, Jolene W. L. ^{[2
]}

Zhang, Zhiwei ^{[2
]}

Sadasivam, Pragalath ^{[1
]}

Tan, Eng King ^{[4
,5
,6
]}

Luthra, Sajinder K. ^{[7
]}

Shingleton, William D. ^{[7
]}

Oh, Steve K. W. ^{[3
]}

Zeng, Li ^{[2
,6
,8
]}

Robins, Edward G. ^{[1
,9
]}

机构：

[1] ASTAR, Singapore Bioimaging Consortium, 11 Biopolis Way,01-02 HELIOS, Singapore 138667, Singapore

[2] Natl Neurosci Inst, Res Dept, Neural Stem Cell Res Lab, 11 Jalan Tan Tock Seng, Singapore 308433, Singapore

[3] ASTAR, Bioproc Technol Inst, 20 Biopolis Way,06-01 Ctr, Singapore 138668, Singapore

[4] Natl Neurosci Inst, Res Dept, SGH Campus, Singapore 169856, Singapore

[5] Natl Neurosci Inst, Dept Neurol, SGH Campus, Singapore 169856, Singapore

[6] DUKE NUS Grad Med Sch, Neurosci & Behav Disorders Program, Singapore 169857, Singapore

[7] GE Healthcare Life Sci, White Lion Rd, Little Chalfont HP7 9LL, Amersham, England

[8] Lee Kong Chian Sch Med, Novena Campus,11 Mandalay Rd, Singapore 308232, Singapore

[9] Natl Univ Singapore, Clin Imaging Res Ctr, Yong Loo Lin Sch Med, Singapore 117599, Singapore

来源：

STEM CELL RESEARCH & THERAPY | 2020年 / 11卷 / 01期

关键词：

Parkinson's disease; Dopaminergic neuron; Cell therapy; PET imaging; DAT; EMBRYONIC STEM-CELLS; SUBSTANTIA-NIGRA; MESSENGER-RNA; VENTRAL MESENCEPHALON; STEREOLOGICAL METHODS; RAT MODEL; TRANSPLANTATION; BRAIN; FETAL; PET;

D O I：

10.1186/s13287-020-01868-4

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Background Significant developments in stem cell therapy for Parkinson's disease (PD) have already been achieved; however, methods for reliable assessment of dopamine neuron maturation in vivo are lacking. Establishing the efficacy of new cellular therapies using non-invasive methodologies will be critical for future regulatory approval and application. The current study examines the utility of neuroimaging to characterise the in vivo maturation, innervation and functional dopamine release of transplanted human embryonic stem cell-derived midbrain dopaminergic neurons (hESC-mDAs) in a preclinical model of PD. Methods Female NIH RNu rats received a unilateral stereotaxic injection of 6-OHDA into the left medial forebrain bundle to create the PD lesion. hESC-mDA cell and sham transplantations were carried out 1 month post-lesion, with treated animals receiving approximately 4 x 10(5)cells per transplantation. Behavioural analysis, [F-18]FBCTT and [F-18]fallypride microPET/CT, was conducted at 1, 3 and 6 months post-transplantation and compared with histological characterisation at 6 months. Results PET imaging revealed transplant survival and maturation into functional dopaminergic neurons. [F-18]FBCTT-PET/CT dopamine transporter (DAT) imaging demonstrated pre-synaptic restoration and [F-18]fallypride-PET/CT indicated functional dopamine release, whilst amphetamine-induced rotation showed significant behavioural recovery. Moreover, histology revealed that the grafted cells matured differently in vivo producing high- and low-tyrosine hydroxylase (TH) expressing cohorts, and only [F-18]FBCTT uptake was well correlated with differentiation. Conclusions This study provides further evidence for the value of in vivo functional imaging for the assessment of cell therapies and highlights the utility of DAT imaging for the determination of early post-transplant cell maturation and differentiation of hESC-mDAs.

引用

页数：14

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