Determination of agmatine in brain and plasma using high-performance liquid chromatography with fluorescence detection

被引:99
作者
Feng, YZ
Halaris, AE
Piletz, JE
机构
[1] UNIV MISSISSIPPI,MED CTR,DEPT PSYCHIAT & HUMAN BEHAV,JACKSON,MS 39216
[2] UNIV MISSISSIPPI,MED CTR,DEPT PHARMACOL & TOXICOL,JACKSON,MS 39216
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 691卷 / 02期
关键词
agmatine; arginine; imidazoline receptors;
D O I
10.1016/S0378-4347(96)00458-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Decarboxylated arginine, agmatine, is a neurotransmitter candidate for imidazoline receptors. A method is described to measure agmatine in rat brain and human plasma by isocratic high-performance liquid chromatography (HPLC) with fluorescence detection and o-phthalaldehyde derivatization. Quantitation is based on the method of additions of internal agmatine spikes. This assay has sensitivity in the low picomole range and a detection limit of 100 fmol. The correlation coefficient for the agmatine standard curve was 0.999+/-0.001 S.D., and intra- and inter-assay C.V.s were less than 8%. The accuracy of our isocratic method compared favorably with a gradient HPLC protocol, originally developed for bacterial agmatine, which we modified for use with tissues. Agmatine concentrations in rat brain were proportioned similarly to the regional distribution of imidazoline-l receptors. These methods can be used as reliable research tools in various biological samples.
引用
收藏
页码:277 / 286
页数:10
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