Adiposoft: automated software for the analysis of white adipose tissue cellularity in histological sections

被引:322
作者
Galarraga, Miguel [1 ,2 ]
Campion, Javier [1 ,3 ,4 ]
Munoz-Barrutia, Arrate [2 ]
Boque, Noemi [3 ,4 ]
Moreno, Haritz [3 ,4 ]
Alfredo Martinez, Jose [3 ,4 ]
Milagro, Fermin [3 ,4 ]
Ortiz-de-Solorzano, Carlos [1 ,2 ]
机构
[1] Univ Navarra, Imaging Unit, E-31080 Pamplona, Spain
[2] Univ Navarra, Canc Imaging Lab, E-31080 Pamplona, Spain
[3] Univ Navarra, Ctr Appl Med Res CIMA, E-31080 Pamplona, Spain
[4] Univ Navarra, Dept Nutr Food Sci Physiol & Toxicol, E-31080 Pamplona, Spain
关键词
fat cell diameter; fat tissue cellularity; adipocyte; quantification; image analysis; software validation; obesity; ADIPOCYTE SIZE; OBESITY;
D O I
10.1194/jlr.D023788
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The accurate estimation of the number and size of cells provides relevant information on the kinetics of growth and the physiological status of a given tissue or organ. Here, we present Adiposoft, a fully automated open-source software for the analysis of white adipose tissue cellularity in histological sections. First, we describe the sequence of image analysis routines implemented by the program. Then, we evaluate our software by comparing it with other adipose tissue quantification methods, namely, with the manual analysis of cells in histological sections (used as gold standard) and with the automated analysis of cells in suspension, the most commonly used method. Our results show significant concordance between Adiposoft and the other two methods. We also demonstrate the ability of the proposed method to distinguish the cellular composition of three different rat fat depots. Moreover, we found high correlation and low disagreement between Adiposoft and the manual delineation of cells. We conclude that Adiposoft provides accurate results while considerably reducing the amount of time and effort required for the analysis.-Galarraga, M., J. Campion, A. Munoz-Barrutia, N. Boque, H. Moreno, J. A. Martinez, F. Milagro, and C. Ortiz-de-Solorzano. Adiposoft: automated software for the analysis of white adipose tissue cellularity in histological sections. J. Lipid Res. 2012. 53: 2791-2796.
引用
收藏
页码:2791 / 2796
页数:6
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